Aibibaihan Matur-Zi, Zhang Juan, Zhu Jun, Wang Guo-Ping, Li Xiao-Jin, Qiu Yuan-Jin, He Wei, Wang Li
Traditional Chinese Medicine Institute, Xinjiang Medical University Urumqi 830000, China.
Institute of Traditional Chinese Medicine and National Medicine of Xinjiang Uyghur Autonomous Region Urumqi 830000,China.
Zhongguo Zhong Yao Za Zhi. 2021 Aug;46(15):3886-3892. doi: 10.19540/j.cnki.cjcmm.20210422.304.
This study established high-performance liquid chromatography(HPLC) fingerprints of Chinese medicines derived from Apocynum venetum and Poacynum pictum in Xinjiang and explored their composition differences with the combination of content determination, similarity analysis, cluster analysis and principal component analysis. The HPLC conditions included Phenomenex Kinetex C_(18) column(4.6 mm ×100 mm, 2.6 μm), acetonitrile-0.01% trifluoroacetic acid aqueous solution as mobile phase, gradient elution, flow rate of 0.6 mL·min~(-1), detection wavelength of 281 nm and column temperature of 25 ℃. The content of chlorogenic acid, quercetin-3-O-sophoroside, rutin, hyperin, isoquercitrin, trifolin and astragalin was determined in 31 batches of medicinal materials, and fingerprint research and chemometric analysis were performed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004 A) and SPSS 21.0. In the Chinese Pharmacopoeia 2020, the quality of Apocyni Veneti Folium is controlled by character identification, microscopic identification, thin layer chromatography identification and quantitative determination of hyperin. There were 21 common peaks of A. venetum and P. pictum in the HPLC fingerprints, 5 of which were identified as chlorogenic acid, hyperin, isoquercitrin, trifolin and astragalin, with their content also determined. Except for 3 batches of medicinal materials, the similarity of other 28 batches was higher than 0.83, indicating good similarity. Two categories were formed in the cluster analysis based on content determination, which showed that some differences existed in similarities between different regions of Xinjiang. The medicinal materials were ranked by quality with principal component analysis, and the results indicated that the top 15 all came from northern Xinjiang. The quality difference of A. venetum and P. pictum had a correlation with the place of origin. This study provides a reference for the analysis and evaluation of A. venetum and P. pictum from different habitats and the selection of introduction and cultivation areas.
本研究建立了新疆罗布麻和大叶白麻药材的高效液相色谱（HPLC）指纹图谱，并结合含量测定、相似度分析、聚类分析和主成分分析，探讨了它们的成分差异。HPLC条件为：菲罗门Kinetex C₁₈色谱柱（4.6 mm×100 mm，2.6 μm），以乙腈-0.01%三氟乙酸水溶液为流动相，梯度洗脱，流速0.6 mL·min⁻¹，检测波长281 nm，柱温25℃。测定了31批药材中绿原酸、槲皮素-3-O-槐糖苷、芦丁、金丝桃苷、异槲皮苷、三叶豆苷和紫云英苷的含量，并采用《中药色谱指纹图谱相似度评价系统》（2004 A版）和SPSS 21.0进行指纹图谱研究和化学计量学分析。《中国药典》2020年版中，罗布麻叶的质量控制采用性状鉴别、显微鉴别、薄层色谱鉴别和金丝桃苷的含量测定。HPLC指纹图谱中罗布麻和大叶白麻有21个共有峰，其中5个被鉴定为绿原酸、金丝桃苷、异槲皮苷、三叶豆苷和紫云英苷，并测定了它们的含量。除3批药材外，其他28批的相似度均高于0.83，表明相似度良好。基于含量测定的聚类分析形成了两类，表明新疆不同地区之间的相似度存在一些差异。通过主成分分析对药材进行质量排序，结果表明前15名均来自新疆北部。罗布麻和大叶白麻的质量差异与产地有关。本研究为不同产地罗布麻和大叶白麻的分析评价及引种栽培区域的选择提供了参考。
