（毛茛科）抗疟原虫活性对致死性鼠疟的作用。

Antiplasmodial potential of (Ranunculaceae) against lethal murine malaria.

机构信息

Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh-160014, India.

出版信息

J Vector Borne Dis. 2020 Jul-Sep;57(3):204-212. doi: 10.4103/0972-9062.311772.

DOI:10.4103/0972-9062.311772

PMID:34472503

Abstract

BACKGROUND AND OBJECTIVES

The emergence of multi-drug resistant (MDR) strains of Plasmodium falciparum highlights the need to develop novel antimalarial drugs. Present study explores the in vivo antiplasmodial activity of ethanol leaf extract of Thalictrum foliolosum (ELETF) against lethal murine malaria.

METHODS

The acute toxicity of the extract was assessed by Limit test of Lorke. The suppressive activity of the extract was evaluated by Peter's 4 day test. In vivo preventive and curative activity of ELETF was assessed by Peter's method and Ryley and Peter's method respectively. Biochemical assays were carried out using standard methods.

RESULTS

ELETF (1000 mg/kg) exhibited considerable in vivo schizontocidal activity with 67.11% chemosuppression on Day 5. The ED of the extract was 579.56 mg/kg. ELETF also showed significant repository activity with 87.70% chemosuppression at 750 mg/kg, which was greater than pyrimethamine (78.78%). ELETF exhibited dose dependent chemosuppression in the curative test with maximum 70.06% chemosuppression (750 mg/kg). Maximum Mean Survival Time (MST) was 19.2±4.60 and 22.66±4.41 days respectively in the suppressive and curative test, which was extremely statistically significant (p<0.0005) in comparison to untreated control which died by Day 9 post inoculation. Biochemical analysis revealed the safety of ELETF to the hepatic and renal functions of the rodent host.

INTERPRETATION & CONCLUSION: The study reports the antiplasmodial potential of Thalictrum foliolosum (ELETF) against Plasmodium berghei infection. The extract can be developed as a phytomedicine against malaria. Alternatively, the active components can be isolated as new lead compounds against the disease.

摘要

背景与目的

疟原虫多药耐药（MDR）株的出现凸显了开发新型抗疟药物的必要性。本研究探讨了云南翠雀叶（ELETF）乙醇提取物对致死性鼠疟的体内抗疟活性。

方法

采用洛克极限试验评估提取物的急性毒性。采用彼得斯 4 天试验评估提取物的抑制活性。采用彼得斯法和赖利-彼得斯法分别评估 ELETF 的体内预防和治疗活性。采用标准方法进行生化测定。

结果

ELETF（1000mg/kg）在第 5 天表现出相当大的体内裂殖体杀伤活性，化学抑制率为 67.11%。提取物的 ED 为 579.56mg/kg。ELETF 还表现出显著的储库活性，在 750mg/kg 时化学抑制率为 87.70%，大于乙胺嘧啶（78.78%）。ELETF 在治疗试验中表现出剂量依赖性的化学抑制作用，最大化学抑制率为 70.06%（750mg/kg）。在抑制和治疗试验中，最大平均存活时间（MST）分别为 19.2±4.60 和 22.66±4.41 天，与未接种对照相比，差异具有统计学意义（p<0.0005），未接种对照在接种后第 9 天死亡。生化分析表明，ELETF 对啮齿动物宿主的肝肾功能是安全的。