Institute of Ophthalmology, School of Medicine, Jinan University, Guangzhou, China.
Department of Ophthalmology, the First Affiliated Hospital, Jinan University, Guangzhou, China.
Invest Ophthalmol Vis Sci. 2021 Sep 2;62(12):1. doi: 10.1167/iovs.62.12.1.
HIV infection is associated with a variety of ocular surface diseases. Understanding the difference of the ocular microbiota between HIV-infected and healthy individuals as well as the influence of antiretroviral therapy will help to investigate the pathogenesis of these conditions.
A cross-sectional study was conducted on subjects including HIV-negative individuals, untreated HIV-infected individuals, and HIV-infected individuals with antiretroviral therapy. Conjunctival microbiota was assessed by bacterial 16S rRNA sequencing of the samples obtained from the conjunctival swab.
The microbial richness in ocular surface was similar in HIV-negative, untreated HIV-positive, and highly active antiretroviral therapy (HAART) subjects. The bacterial compositions were similar in the two HIV infection groups but were significantly different from the HIV-negative group. HAART changed the beta diversity of bacterial community as determined by Shannon index. CD4+ T cell count had no significant influence on the diversity of ocular microbiota in HIV-infected individuals.
The data revealed the compositional and structural difference in conjunctival microbial community in subjects with and without HIV infection, indicating that HIV infection or its treatment, may contribute to ocular surface dysbiosis.
HIV 感染与多种眼表面疾病有关。了解 HIV 感染者和健康个体之间眼表微生物组的差异，以及抗逆转录病毒治疗的影响，有助于研究这些疾病的发病机制。
对包括 HIV 阴性个体、未经治疗的 HIV 感染者和接受抗逆转录病毒治疗的 HIV 感染者在内的受试者进行了一项横断面研究。通过对结膜拭子样本进行细菌 16S rRNA 测序来评估结膜微生物组。
在 HIV 阴性、未经治疗的 HIV 阳性和高效抗逆转录病毒治疗（HAART）受试者中，眼表微生物丰富度相似。两组 HIV 感染患者的细菌组成相似，但与 HIV 阴性组有显著差异。HAART 改变了 Shannon 指数确定的细菌群落的β多样性。CD4+T 细胞计数对 HIV 感染者眼表微生物多样性没有显著影响。
数据显示了 HIV 感染和未感染个体结膜微生物群落的组成和结构差异，表明 HIV 感染或其治疗可能导致眼表面微生态失调。
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