National Institute for Laser, Plasma, and Radiation Physics, Lasers Department, 409 Atomistilor, 077125 Magurele, Ilfov, Romania.
National Institute for Laser, Plasma, and Radiation Physics, Lasers Department, 409 Atomistilor, 077125 Magurele, Ilfov, Romania.
J Chromatogr A. 2021 Oct 11;1655:462488. doi: 10.1016/j.chroma.2021.462488. Epub 2021 Aug 23.
A densitometry method based on steady-state and time-resolved fluorescence assessments for thioridazine and its photoproducts applied on HPTLC plates has been developed. The excitation source was a picosecond diode laser emitting at 375 nm. This method was used for the analysis of the photoproducts resulted from thioridazine irradiation with 266 nm nanosecond-pulsed laser. The validation of the developed method was performed for thioridazine in terms of linearity, precision, limits of detection and quantification. Furthermore, analysis of the photoproducts of irradiated thioridazine was performed by steady-state and time-resolved fluorescence. The fluorescence spectra and fluorescence lifetime of each photoproduct were obtained and the horizontal chromatograms of fluorescence maxima were generated.
已经开发出一种基于稳态和时间分辨荧光评估的、用于噻吨嗪及其光产物的密度测定方法，应用于 HPTLC 板上。激发源是发射波长为 375nm 的皮秒二极管激光。该方法用于分析噻吨嗪用 266nm 纳秒脉冲激光辐照产生的光产物。针对噻吨嗪，从线性、精密度、检测限和定量限方面对所开发方法进行了验证。此外，通过稳态和时间分辨荧光对辐照噻吨嗪的光产物进行了分析。获得了每个光产物的荧光光谱和荧光寿命，并生成了荧光极大值的水平色谱图。
