Laser Department, National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor, 077125 Magurele, Ilfov, Romania.
Faculty of Physics, University of Bucharest, 405 Atomistilor, 077125 Magurele, Romania.
Molecules. 2021 Apr 16;26(8):2324. doi: 10.3390/molecules26082324.
Ciprofloxacin is one of the most prescribed antibiotics in treating bacterial infections, becoming an important pollutant of the wastewaters. Moreover, ciprofloxacin is hard to be destroyed by conventional water treatment processes; therefore, efficient treatments to destroy it are needed in water decontamination. This study offers insights into the performance of 266 nm laser beams on the photodegradation of ciprofloxacin. An Nd:YAG laser was used that emitted 266 nm at an energy of 6.5 mJ (power of 65 mW) and ciprofloxacin water solutions were irradiated up to 240 min. The irradiated solutions were investigated by UV-Vis and FTIR absorption spectroscopy, pH assay, and laser-induced fluorescence. An HPTLC densitometer was used to characterize the laser-induced fluorescence and fluorescence lifetime of photodegradation products. The UV-Vis absorption, FTIR, and laser-induced fluorescence spectra showed the degradation of ciprofloxacin. Moreover, HPTLC densitometry offered the fluorescence and fluorescence lifetime of ciprofloxacin and its three photoproducts as well as their relative quantification. From the FTIR spectra, the molecular structure of two out of three photoproducts was proposed. In conclusion, the laser irradiation method provided the efficient photodegradation of ciprofloxacin, whereas the analytical techniques offered the proper means to monitor the process and detect the obtained photoproducts.
环丙沙星是治疗细菌感染最常开的抗生素之一，成为废水中的重要污染物。此外，环丙沙星很难被常规水处理工艺破坏；因此，在水净化中需要有效的处理方法来破坏它。本研究探讨了 266nm 激光束对环丙沙星光降解性能的影响。使用 Nd:YAG 激光器发射 266nm 的激光，能量为 6.5mJ（功率为 65mW），将环丙沙星水溶液辐照长达 240 分钟。通过紫外-可见和傅里叶变换红外吸收光谱、pH 测定和激光诱导荧光对辐照溶液进行了研究。使用 HPTLC 密度计对激光诱导荧光和光降解产物的荧光寿命进行了表征。紫外-可见吸收、傅里叶变换红外和激光诱导荧光光谱表明环丙沙星发生了降解。此外，HPTLC 密度计提供了环丙沙星及其三种光产物的荧光和荧光寿命及其相对定量。从傅里叶变换红外光谱中，提出了三种光产物中的两种的分子结构。总之，激光辐照方法提供了环丙沙星的有效光降解，而分析技术则提供了监测过程和检测所得光产物的适当手段。
