School of Public Health, Xinxiang Medical University, Xinxiang 453003, China.
Henan Provincial Key Laboratory of Children's Genetics and Metabolic Diseases/Henan Neural Development Engineering Research Center for Children, Department of Nephrology and Rheumatology, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, China.
Sci Total Environ. 2022 Jan 1;802:149914. doi: 10.1016/j.scitotenv.2021.149914. Epub 2021 Aug 26.
The increasing production and application of graphene oxide (GO, a popular carbon nanomaterial), makes their release into aqueous environment inevitably. The capability of GO to enhance the toxicity of background contaminants has been widely concerned. However, the effect of GO on heavy metal accumulation in fish embryos remains unclear. Here, we show that GO-promoted chromium (Cr) uptake by zebrafish embryos with multiple effects. The adsorption accelerated the aggregation and settlement of Cr-adsorbed GO and decreased the Cr concentration in the upper water, which enhanced the interaction of chorions and contaminants (Cr, GO and Cr-adsorbed GO). In the presence of GO, the Cr content in chorions and intra-chorion embryos was increased by four times and 57% respectively, compared to that of the single Cr exposure. Furthermore, GO+Cr increased the oxygen consumption rates, embryonic acid extrusion rates and ATP production, induced more serious oxidative stress, and disturbed amino acid metabolism, fatty acid metabolism and TCA cycle. These findings provide new insights into the effect of GO on heavy metal bioaccumulation and toxicity during embryogenesis.
氧化石墨烯（GO，一种常见的碳纳米材料）的产量和应用不断增加，使其不可避免地释放到水环境中。GO 增强背景污染物毒性的能力已引起广泛关注。然而，GO 对鱼类胚胎中重金属积累的影响尚不清楚。在这里，我们表明 GO 以多种方式促进了斑马鱼胚胎对铬（Cr）的吸收。吸附加速了吸附 Cr 的 GO 的聚集和沉降，并降低了上层水中的 Cr 浓度，从而增强了绒毛膜和污染物（Cr、GO 和吸附 Cr 的 GO）之间的相互作用。在 GO 的存在下，与单独的 Cr 暴露相比，绒毛膜和绒毛膜内胚胎中的 Cr 含量分别增加了四倍和 57%。此外，GO+Cr 增加了耗氧量、胚胎酸外排率和 ATP 生成，诱导更严重的氧化应激，并扰乱了氨基酸代谢、脂肪酸代谢和 TCA 循环。这些发现为 GO 在胚胎发生过程中对重金属生物积累和毒性的影响提供了新的见解。
