Center for Biofluid and Biomimic Research, Pohang University of Science and Technology (POSTECH), Pohang 37673, South Korea.
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, South Korea.
Sci Total Environ. 2019 Jul 10;673:810-820. doi: 10.1016/j.scitotenv.2019.04.082. Epub 2019 Apr 8.
Graphene oxide (GO) has wide engineering applications in various areas, including electronics, energy storage, pharmaceuticals, nanomedicine, environmental remediation and biotechnology, because of its unique physico-chemical properties. In the present study, the risk-related information of GO was evaluated to examine the potential ecological and health risks of developmental toxicity. Although the overall developmental toxicity of GO has been well characterized in zebrafish, however, its release effect at a certain concentration of living organisms with specific cardiovascular defects remains largely elusive. Therefore, this study was conducted to further evaluate the toxicity of GO on embryonic development and cardiovascular defects in zebrafish embryos used as an in-vivo animal model. As a result, the presence of GO at a small concentration (0.1-0.3 mg/mL) does not affect the embryonic development. However, GO at higher concentrations (0.4-1 mg/mL) induces significant embryonic mortality, increase heartbeat, delayed hatching, cardiotoxicity, cardiovascular defects, retardation of cardiac looping, increased apoptosis and decreased hemoglobinization. These results provide valuable information that can be used to study the eco-toxicological effects of GO for assessing its bio-safety according to environmental concentration. In addition, the present results would also be usefully utilized for understanding the environmental risks associated with GO on human health in general.
氧化石墨烯（GO）由于其独特的物理化学性质，在电子、储能、制药、纳米医学、环境修复和生物技术等各个领域都有广泛的工程应用。在本研究中，评估了 GO 的风险相关信息，以研究其潜在的生态和健康发育毒性风险。尽管 GO 在斑马鱼中的整体发育毒性已得到充分表征，但其在具有特定心血管缺陷的生物体内特定浓度下的释放效应在很大程度上仍难以捉摸。因此，本研究旨在进一步评估 GO 对斑马鱼胚胎发育和心血管缺陷的毒性，斑马鱼胚胎可用作体内动物模型。结果表明，低浓度（0.1-0.3mg/mL）GO 的存在并不影响胚胎发育。然而，高浓度（0.4-1mg/mL）GO 诱导显著的胚胎死亡率、增加心跳、延迟孵化、心脏毒性、心血管缺陷、心脏环化延迟、凋亡增加和血红蛋白化减少。这些结果提供了有价值的信息，可用于根据环境浓度研究 GO 的生态毒理学效应，以评估其生物安全性。此外，本研究结果还可用于一般了解 GO 对人类健康相关的环境风险。
