Mohammad Samira Metwally, Ali Magda, Abdel-Rahman Sara A, Moustafa Raghda Abdelrahman, Salama Marwa A
Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
J Parasit Dis. 2021 Sep;45(3):746-753. doi: 10.1007/s12639-020-01345-y. Epub 2021 Feb 6.
Immunocompromised individuals especially children with cancer are at risk for acquiring cryptosporidiosis, which can result in severe morbidity and mortality. This work was conducted to evaluate the prevalence of parasite and its genotypes in children with cancer. Stool specimens were collected from 145 children in the Oncology unit of Pediatric Department, Zagazig University Hospital, Sharqiyah province, Egypt. infection was evaluated using modified Ziehl-Neelsen (MZN) staining and nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The prevalence of infection in oncological children was 29.7% using microscopy and 25.5% using nested PCR. Genotypic characterization showed that 23 (62.2%) had , 11 (29.7%) , and 3 specimens (8.1%) were mixed infection of both genotypes. Cryptosporidiosis was significantly associated with diarrhea. However, no statistically significant difference was detected between the age, gender, residency, animal contact and malignancy type concerning to infection. This study concluded that is a prevailing opportunistic parasite among children with cancer. It should be considered in oncological patients especially those suffering from diarrhea which requires proper management to reduce its complications.
免疫功能低下的个体，尤其是患癌症的儿童，有感染隐孢子虫病的风险，这可能导致严重的发病和死亡。开展这项工作是为了评估患癌症儿童中该寄生虫及其基因型的流行情况。从埃及谢赫村省扎加济格大学医院儿科肿瘤科的145名儿童中采集粪便样本。使用改良齐-尼氏（MZN）染色和巢式聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析评估感染情况。显微镜检查显示肿瘤患儿的感染率为29.7%，巢式PCR检测为25.5%。基因型特征显示，23例（62.2%）为微小隐孢子虫，11例（29.7%）为人隐孢子虫，3份样本（8.1%）为两种基因型的混合感染。隐孢子虫病与腹泻显著相关。然而，在年龄、性别、居住地、动物接触和恶性肿瘤类型方面，未检测到与隐孢子虫感染相关的统计学显著差异。本研究得出结论，隐孢子虫是患癌症儿童中一种常见的机会性寄生虫。在肿瘤患者中应予以考虑，尤其是那些患有腹泻的患者，这需要适当管理以减少其并发症。
