接受化疗的儿科肿瘤患儿的肠道寄生虫感染：意外的低患病率。
Intestinal parasitism in pediatric oncology children receiving chemotherapy: unexpected low prevalence.
作者信息
El-Badry Ayman A, El Sayed Samar S, Hussein Rafiaa R, Said Youssef M, Al-Antably Abeer S, Hassan Marwa A
机构信息
Department of Microbiology-Medical Parasitology Section, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Department of Parasitology, Faculty of Medicine for Girls, Al-Azhar University, Egypt.
出版信息
Heliyon. 2019 Aug 12;5(8):e02228. doi: 10.1016/j.heliyon.2019.e02228. eCollection 2019 Aug.
BACKGROUND
Children with underlying malignancies and those on chemotherapy are at risk for having intestinal parasitic infections, which can lead to a severe course and death. This cross-sectional study was done to assess the copro-parasitological and copro-molecular prevalence of entero-parasites in children with malignancies and those on chemotherapy.
PROCEDURE
Stool samples were collected from 137 Egyptian hospitalized cancerous children with different malignancies in the National Cancer Institute, and receiving chemotherapy.Faecal samples were examined microscopically. Genomic copro-DNA was extracted from fecal samples and amplified by 3 separate nPCR assays targeting , and complex.
RESULT
The overall prevalence of enteroparasites was 6.6 % (9 cases). Only copro-DNA was encountered in 2 (1.4%) faecal samples of patients. Coproscopy detected parasites in 7 cases: in 5 cases (3.6%), in 1 case (0.7%) and in 1 case (0.7%).
CONCLUSION
Low prevalence may be due to patient's use of prophylactic anti-parasitic and anti-fungal drugs, a standard protocol, basic hygienic practices and good nursing all of which are preventive against enteroparasites transmission. Among studied variables only diarrhoeic individuals who had a solid tumor, and soft/liquid stool with mucus and blood were predictors of intestinal parasitism.
背景
患有潜在恶性肿瘤的儿童以及接受化疗的儿童有感染肠道寄生虫的风险，这可能导致病情严重甚至死亡。本横断面研究旨在评估恶性肿瘤患儿及接受化疗患儿肠道寄生虫的粪便寄生虫学和粪便分子流行率。
程序
从国家癌症研究所137名患有不同恶性肿瘤且正在接受化疗的埃及住院癌症患儿中采集粪便样本。对粪便样本进行显微镜检查。从粪便样本中提取基因组粪便DNA，并通过针对、和复合体的3种独立巢式PCR检测进行扩增。
结果
肠道寄生虫的总体流行率为6.6%（9例）。仅在2例（1.4%）患者的粪便样本中检测到粪便DNA。粪便检查在7例中检测到寄生虫：5例（3.6%）为，1例（0.7%）为，1例（0.7%）为。
结论
低流行率可能归因于患者使用预防性抗寄生虫和抗真菌药物、标准方案、基本卫生习惯以及良好护理，所有这些都可预防肠道寄生虫传播。在所研究的变量中，只有患有实体瘤且粪便为软便/稀便并伴有黏液和血液的腹泻个体是肠道寄生虫感染的预测因素。
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