Gorka C, Lawrence J J
Nucleic Acids Res. 1979 Sep 25;7(2):347-59. doi: 10.1093/nar/7.2.347.
Rat liver chromatin was digested with micrococcal nuclease to various extents and fractionated into nucleosomes, di and trimers of nucleosomes on an isokinetic sucrose gradient. In conditions under which degradation of linker DNA within the particles was limited, the electrophoretic analysis of the histone content showed that the overall content of H1 histone increased from nucleosomes to higher order oligomers. Moreover, the histone H1 subfractions were found unevenly distributed among the chromatin subunits, one of them, H1--3 showing most variation. A more regular distribution of these subfractions was found in subunits obtained from a more extended digestion level of chromatin. It is suggested that the H1 subfractions differ in the protection they confer upon DNA.
用微球菌核酸酶将大鼠肝脏染色质消化至不同程度，并在等密度蔗糖梯度上分离成核小体、核小体二聚体和三聚体。在颗粒内连接DNA降解受限的条件下，对组蛋白含量的电泳分析表明，H1组蛋白的总含量从核小体到高阶寡聚体逐渐增加。此外，发现组蛋白H1亚组分在染色质亚基中分布不均，其中一个亚组分H1-3变化最大。在从染色质消化程度更高的水平获得的亚基中，发现这些亚组分的分布更规则。提示H1亚组分在对DNA的保护作用上存在差异。
