Laboratory for Molecular Electronics and Photonics, KU Leuven, Celestijnenlaan 200D, 3001, Leuven, Belgium.
Laboratory of Soft Matter and Biophysics, KU Leuven, Celestijnenlaan 200D, 3001, Leuven, Belgium.
Biosens Bioelectron. 2021 Dec 15;194:113577. doi: 10.1016/j.bios.2021.113577. Epub 2021 Aug 27.
To overcome the problems of refractive index matching and increased disorder when working with traditional heterostructure colloidal photonic crystals (CPCs) with dual or multiple photonic bandgaps (PBGs) for fluorescence enhancement in water, we propose the use of a chemical heterostructure in hollow sphere CPCs (HSCPCs). A partial chemical modification of the HSCPC creates a large contrast in wettability to induce the heterostructure, while the hollow spheres increase the refractive index difference when used in aqueous environment. With the platform, fluorescence enhancement reaches around 160 times in solution, and 72 times (signal-to-background ratio ~7 times) in cells during proof-of-concept live cardiomyocyte contractility experiments. Such photonic platform can be further exploited for chemical sensing, bioassays, and environmental monitoring. Moreover, the introduction of chemical heterostructures provides new design principles for functionalized photonic devices.
为了解决在水中增强荧光时使用具有双或多个光子带隙（PBG）的传统异质结构胶体光子晶体（CPC）的折射率匹配和无序增加的问题，我们提出在中空球 CPC（HSCPC）中使用化学异质结构。通过对 HSCPC 进行部分化学修饰，可以在润湿性方面产生较大的对比度，从而诱导异质结构，而中空球在用于水相环境时会增加折射率差异。在该平台上，在溶液中荧光增强达到约 160 倍，在活心肌细胞收缩性实验的概念验证过程中在细胞中达到 72 倍（信号-背景比约 7 倍）。该光子平台可进一步用于化学传感、生物测定和环境监测。此外，化学异质结构的引入为功能化光子器件提供了新的设计原则。
