School of Optoelectronic Engineering, Chongqing University of Posts and Telecommunications, Chongqing, 400065, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China; Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714, China.
Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China; Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714, China.
Biosens Bioelectron. 2021 Dec 15;194:113602. doi: 10.1016/j.bios.2021.113602. Epub 2021 Aug 30.
Inhibition of HIV-1 protease (PR) activity is realized by exposure to Co γ-radiation. The radiation effects on enzyme kinetics of HIV-1 PR are subsequently monitored using nanopore sensor. Substantial loss of proteolytic efficiency towards GagPol polypeptide is observed due to the radiation treatment. Results shows ~50% of GagPol polypeptide was not involved in HIV-1 PR proteolysis by exposure to ultra-low intensity of γ-radiation (0.1K Gy), and the values reach to over 90% with high γ-ray treatment. Besides, the inactivation effect is also verified in blood samples which contain the virus protease. Our finding provides the potential benefits of γ-radiation to inactivate viral proteinic function, and might be a complementary to the designation of HIV-1 PR inhibitors.
通过暴露于 Co γ 射线来实现对 HIV-1 蛋白酶（PR）活性的抑制。随后使用纳米孔传感器监测 HIV-1 PR 的酶动力学的辐射效应。由于辐射处理，观察到针对 GagPol 多肽的酶切效率明显降低。结果表明，由于暴露于超低强度γ射线（0.1K Gy），约有 50%的 GagPol 多肽未参与 HIV-1 PR 的蛋白水解，而在高γ射线处理时，其值达到 90%以上。此外，还在含有病毒蛋白酶的血液样本中验证了失活效果。我们的发现为γ射线灭活病毒蛋白功能提供了潜在的益处，并且可能是 HIV-1 PR 抑制剂设计的补充。
