通过纳米孔分析对HIV-1蛋白酶活性进行无标记实时测量。
Real-time label-free measurement of HIV-1 protease activity by nanopore analysis.
作者信息
Wang Liang, Han Yujing, Zhou Shuo, Guan Xiyun
机构信息
Department of Biological and Chemical Sciences, Illinois Institute of Technology, Chicago, IL 60616, United States.
Department of Biological and Chemical Sciences, Illinois Institute of Technology, Chicago, IL 60616, United States.
出版信息
Biosens Bioelectron. 2014 Dec 15;62:158-62. doi: 10.1016/j.bios.2014.06.041. Epub 2014 Jun 25.
Abstract
A label-free method for the measurement of the activity of HIV-1 protease is developed by real-time monitoring of the cleavage of a peptide substrate by HIV-1 protease in a nanopore. The method is rapid and sensitive: picomolar concentrations of HIV-1 protease could be detected in ~10 min. Simulated clinical samples are analyzed, and the activity of HIV-1 protease could be accurately detected. Our developed nanopore sensor design strategy should find useful applications in the development of stochastic sensors for other proteases of medical, pharmaceutical, and biological importance.
摘要
通过实时监测纳米孔中HIV-1蛋白酶对肽底物的切割，开发了一种用于测量HIV-1蛋白酶活性的无标记方法。该方法快速且灵敏：在约10分钟内可检测到皮摩尔浓度的HIV-1蛋白酶。对模拟临床样本进行了分析，并且能够准确检测HIV-1蛋白酶的活性。我们开发的纳米孔传感器设计策略应在开发用于医学、制药和生物学上具有重要意义的其他蛋白酶的随机传感器方面找到有用的应用。
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