Liu Jiayu, Quan Zhen, Gao Yingying, Wu Xiaohou, Zheng Yongbo
Department of Urology Surgery, The First Affiliated Hospital of Chongqing Medical University.No.1, Youyi Road, Chongqing, 400016, PR China.
Department of Urology Surgery, The First Affiliated Hospital of Chongqing Medical University.No.1, Youyi Road, Chongqing, 400016, PR China.
Biochem Biophys Res Commun. 2021 Oct 22;576:73-79. doi: 10.1016/j.bbrc.2021.08.078. Epub 2021 Aug 28.
MicroRNA-199b-3p (miR-199b-3p) plays a crucial role in the malignant development of various cancers, but little known in prostate cancer (PCa). The aim of our study was to demonstrate the function of miR-199b-3p in PCa.
Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect miR-199b-3p expression in PCa and benign prostatic hyperplasia (BPH) tissue samples. In addition, we examined the relationship between the poor prognosis in PCa and miR-199b-3p. Western blot was used to analyze the expression of Phospholipase Cε (PLCε). CCK8 and colony-forming assays were applied to detect the proliferation of PCa. EdU assay is used to detect PCa cells uptake of EdU. Luciferase reporter assay was applied to analyze the binding between miR-199b-3p and PLCε.
It has been shown that miR-199b-3p in PCa was significantly lower than that in benign prostatic hyperplasia and correlated with poor prognosis. Meanwhile, upregulation of miR-199b-3p can prominently inhibit the proliferation of PCa cells, while its down-regulation triggered opposite result. PLCε was identified as the downstream binding target gene and negatively associated with that of miR-199b-3p.
miR-199b-3p suppresses malignant proliferation by inhibiting PLCε in prostate cancer in vitro and vivo.
微小RNA-199b-3p（miR-199b-3p）在多种癌症的恶性发展中起关键作用，但在前列腺癌（PCa）中的情况鲜为人知。我们研究的目的是证明miR-199b-3p在PCa中的功能。
采用定量实时聚合酶链反应（RT-qPCR）检测PCa和良性前列腺增生（BPH）组织样本中miR-199b-3p的表达。此外，我们研究了PCa预后不良与miR-199b-3p之间的关系。采用蛋白质免疫印迹法分析磷脂酶Cε（PLCε）的表达。应用CCK8和集落形成试验检测PCa的增殖。EdU试验用于检测PCa细胞对EdU的摄取。采用荧光素酶报告基因试验分析miR-199b-3p与PLCε之间的结合。
结果显示，PCa中miR-199b-3p显著低于良性前列腺增生，且与预后不良相关。同时，上调miR-199b-3p可显著抑制PCa细胞的增殖，而下调则产生相反的结果。PLCε被确定为下游结合靶基因，且与miR-199b-3p呈负相关。
miR-199b-3p在体外和体内通过抑制PLCε抑制前列腺癌的恶性增殖。
