Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, PR China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, PR China.
Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, PR China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, PR China; Collaborative Innovation Center of Water Pollution Control and Water Quality Security Assurance of Shaanxi Province, Xi'an University of Architecture and Technology, Xi'an 710055, PR China.
J Hazard Mater. 2022 Feb 5;423(Pt A):127102. doi: 10.1016/j.jhazmat.2021.127102. Epub 2021 Sep 1.
In this work, sequential applications of light-emitting diodes (UV-LEDs) with two wavelengths and chlorine (Cl) were performed for fungal spores disinfection: UV-Cl, Cl-UV, UV/Cl-UV, UV-UV/Cl, Cl-UV/Cl-Cl. Overall comparisons of the sequential processes with respect to the inhibitory effect on photoreactivation were also evaluated. According to the evaluation of culturability and membrane permeability, inactivation of fungal spores by UV was not enhanced by prior or post exposure to Cl, but in the UV/Cl process with pre or post UV treatment, the inactivation efficiency was greatly enhanced. Take P. polonicum for example, pre-treatments by UV and UV (40 mJ/cm) caused the log count reduction (LCR) of 1.05 log and 0.95 log, then the followed UV/Cl and UV/Cl at the same UV fluence caused additional LCR of 1.80 log and 2.00 log. The permeabilization of P. polonicum was also accelerated in the processes of UV/Cl-UV and UV-UV/Cl, especially at the wavelength of 280 nm. In the sequential processes, especially those containing UV/Cl or at the wavelength of 280 nm, could promote the formation of intracellular reactive oxygen species (ROS), thus leading to more severe damage to the spores as reflected in the culturability reduction, membrane permeability and inhibition of photoreactivation.
在这项工作中，采用两种波长的发光二极管（UV-LEDs）和氯（Cl）对真菌孢子进行了顺序应用以进行消毒：UV-Cl、Cl-UV、UV/Cl-UV、UV-UV/Cl、Cl-UV/Cl-Cl。还对顺序过程相对于光复活抑制的总体比较进行了评估。根据可培养性和膜通透性的评估，先前或随后暴露于 Cl 并没有增强 UV 对真菌孢子的灭活作用，但在具有预先或随后的 UV 处理的 UV/Cl 过程中，灭活效率大大提高。以 P. polonicum 为例，UV 和 UV（40 mJ/cm）的预处理导致对数减少（LCR）分别为 1.05 log 和 0.95 log，然后相同 UV 剂量的 UV/Cl 和 UV/Cl 分别导致额外的 LCR 为 1.80 log 和 2.00 log。在 UV/Cl-UV 和 UV-UV/Cl 过程中，P. polonicum 的通透性也得到了加速，尤其是在 280nm 的波长下。在顺序过程中，特别是那些含有 UV/Cl 或在 280nm 的波长下，会促进细胞内活性氧物质（ROS）的形成，从而导致孢子受到更严重的损伤，这反映在可培养性降低、膜通透性和光复活抑制上。
