College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
Key Laboratory of Fermentation Engineering, Ministry of Education, Hubei University of Technology, Wuhan 430068, China; National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, China; Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China; School of Biological Engineering and Food, Hubei University of Technology, Wuhan 430068, China.
Food Chem. 2022 Feb 1;369:130896. doi: 10.1016/j.foodchem.2021.130896. Epub 2021 Aug 19.
As a typical representative of polymethoxylated flavones, nobiletin (NOB) is beneficial to health but hard to be processed, stored, and absorbed, due to its hydrophobicity and crystallinity. Herein, we developed a stabilization system based on an efficient manufacturing procedure of NOB nanocrystal by anti-solvent method combined with ultrasonic treatment. Metal-phenolic networks composed of tannic acid and metal ions were introduced to conformally coat on formed nanocrystal for further stabilization. From the results, the size and morphology of the prepared particles could be altered by the amount, ratio, and kind of the coating materials. The optimized samples could be redispersed after centrifugation, and keep stable at 4 ℃ for at least 120 days. Moreover, they possessed higher acid stability and more effective release than the control sample during the in vitro digestion experiment. Therefore, this work provided a promising idea for overcoming storage and delivery obstacle of hydrophobic crystalline bioactive components.
作为多甲氧基黄酮的典型代表，诺必特（NOB）由于其疏水性和结晶性，对健康有益，但难以加工、储存和吸收。在此，我们开发了一种基于反溶剂法结合超声处理的高效制造 NOB 纳米晶体的稳定系统。金属-多酚网络由单宁酸和金属离子组成，被引入到形成的纳米晶体上进行进一步的稳定。结果表明，通过涂层材料的用量、比例和种类可以改变所制备颗粒的大小和形态。优化后的样品在离心后可以重新分散，并在 4℃下至少稳定 120 天。此外，与对照样品相比，它们在体外消化实验中具有更高的酸稳定性和更有效的释放。因此，这项工作为克服疏水性结晶生物活性成分的储存和输送障碍提供了一个有前途的思路。
