Landscape Architecture School, Beijing Forestry University, No.35, Tsinghua East Road, Haidian District, Beijing, China.
Plant Sci. 2021 Oct;311:110992. doi: 10.1016/j.plantsci.2021.110992. Epub 2021 Jul 13.
Previous studies demonstrated that flower opening time (FOT) is a stable trait and precisely controlled by a circadian clock responsive to the environment. It plays a vital role in improving fertility. Hemerocallis spp. has different FOTs divided into two types: nocturnal and diurnal. To explore the regulatory mechanisms of their FOTs, we carried out a transcriptome sequencing experiment at different developmental stages of an F1 population with different FOTs. 55,883 unigenes were obtained, and 9234 differential genes were identified. Co-expression was analyzed by K-means clustering and weighted gene co-expression network analysis. Results showed that after entering reproductive growth, two FOT types of Hemerocallis had increased expression of genes related to photosynthetic metabolism and sensitivity to environmental response such as light and hormone signal transmission. Circadian rhythm-related activities were enriched in hub genes during the flowering stage. Genes showing differential expression between the two Hemerocallis groups were related to environmental response and photosynthesis pathways. Putative circadian clock genes displayed differences in expression across the flower opening stage in both groups of Hemerocallis. Twenty-three key circadian clock genes were identified, which related to sensitivity to light signal input and gating. These genes might closely relate to FOTs in Hemerocallis.
先前的研究表明，开花时间（FOT）是一个稳定的特征，并且受到环境响应的生物钟精确控制。它在提高生育能力方面起着至关重要的作用。萱草属植物具有不同的 FOT，分为两类：夜间型和昼间型。为了探索其 FOT 的调控机制，我们在具有不同 FOT 的 F1 群体的不同发育阶段进行了转录组测序实验。获得了 55883 个基因，鉴定了 9234 个差异基因。通过 K-means 聚类和加权基因共表达网络分析进行共表达分析。结果表明，进入生殖生长后，两种 FOT 类型的萱草增加了与光合作用代谢以及对光和激素信号转导等环境响应敏感性相关的基因表达。在开花阶段，生物钟相关活动在枢纽基因中富集。两组萱草之间差异表达的基因与环境响应和光合作用途径有关。在两组萱草的开花阶段，推定的生物钟基因在表达上存在差异。鉴定出 23 个关键的生物钟基因，它们与光信号输入和门控的敏感性有关。这些基因可能与萱草的 FOT 密切相关。
