De Nys Siemon, Putzeys Eveline, Duca Radu Corneliu, Vervliet Philippe, Covaci Adrian, Boonen Imke, Elskens Marc, Vanoirbeek Jeroen, Godderis Lode, Van Meerbeek Bart, Van Landuyt Kirsten L
KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & University Hospitals Leuven (UZ Leuven), Dentistry, Leuven, Belgium.
Environment and Health, Department of Public Health and Primary Care, KU Leuven, Kapucijnenvoer 35, 3000 Leuven, Belgium; Unit Environmental Hygiene and Human Biological Monitoring, Department of Health Protection, National Health Laboratory (LNS), 3555 Dudelange, Luxembourg.
Dent Mater. 2021 Oct;37(10):1561-1568. doi: 10.1016/j.dental.2021.08.005. Epub 2021 Sep 3.
BPA release from composites on the short term has been reported in several in-vitro and in-vivo studies. However, it remains unclear whether these materials also leach BPA on the long term. Even though composites may release various (BPA-based) methacrylate monomers up to one year, quantitative data about BPA have not been reported due to the lack of a sensitive method to accurately quantify low levels of BPA. In this context, the aim of the study was to quantify the one-year release of BPA with an optimized analytical method.
Composite disks (n = 6, 6 mm diameter and 2 mm height) from four commercial materials (G-ӕnial Posterior, Venus, Ceram.x mono and Filtek Supreme XTE) were immersed in 1 mL of water or ethanol as extraction solvent and stored in the dark at 37 °C. The extraction solvent was renewed weekly for a period of 52 weeks. Samples were derivatized with pyridine-3-sulfonyl chloride before analysis with ultra-pressure liquid chromatography tandem mass spectrometry (UPLC-MS/MS).
Derivatizing BPA increased the sensitivity of the analytical method and allowed accurate quantification of very low levels of BPA (i.e. 0.78 pmol BPA). BPA eluted continuously in ethanol from all four tested composites over a period of one year. BPA elution was clearly higher when ethanol was used as extraction solution. In water, BPA eluted could be detected up to one year, but levels could not be accurately quantified anymore after several weeks.
Composites can be considered as a potential long-term source of BPA, and thus should not be neglected when assessing the overall exposure to endocrine disrupting chemicals.
多项体外和体内研究报告了复合材料在短期内双酚A（BPA）的释放情况。然而，这些材料长期是否也会渗出双酚A仍不清楚。尽管复合材料可能在长达一年的时间内释放各种（基于双酚A的）甲基丙烯酸酯单体，但由于缺乏准确量化低水平双酚A的灵敏方法，尚未有关于双酚A的定量数据报告。在此背景下，本研究的目的是用优化的分析方法量化双酚A一年的释放量。
将来自四种商用材料（G-ӕnial Posterior、Venus、Ceram.x mono和Filtek Supreme XTE）的复合盘（n = 6，直径6毫米，高2毫米）浸入1毫升水或乙醇作为萃取溶剂中，并在37°C黑暗条件下储存。萃取溶剂每周更换一次，持续52周。在采用超高压液相色谱串联质谱（UPLC-MS/MS）分析之前，样品用吡啶-3-磺酰氯进行衍生化处理。
双酚A衍生化提高了分析方法的灵敏度，能够准确量化极低水平的双酚A（即0.78皮摩尔双酚A）。在一年的时间里，所有四种测试复合材料中的双酚A都在乙醇中持续洗脱。当使用乙醇作为萃取溶液时，双酚A的洗脱量明显更高。在水中，双酚A的洗脱在长达一年的时间内都能被检测到，但几周后就无法再准确量化其含量。
复合材料可被视为双酚A的潜在长期来源，因此在评估内分泌干扰化学物质的总体暴露时不应被忽视。
