Jiangxi Key Laboratory for Post-Harvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.
Department of Physiology and Biochemistry, Cholistan University of Veterinary and Animal Sciences (CUVAS), Bahawalpur, Punjab 63100, Pakistan.
Oxid Med Cell Longev. 2021 Aug 25;2021:6072631. doi: 10.1155/2021/6072631. eCollection 2021.
Solar ultraviolet radiation A (UVA, 320-400 nm) is a significant risk factor leading to various human skin conditions such as premature aging or photoaging. This condition is enhanced by UVA-mediated iron release from cellular iron proteins affecting huge populations across the globe.
Quercetin-loaded zinc oxide nanoparticles (quercetin@ZnO NPs) were prepared to examine its cellular iron sequestration ability to prevent the production of reactive oxygen species (ROS) and inflammatory responses in HaCaT cells.
Quercetin@ZnO NPs were synthesized through a homogenous precipitation method, and the functional groups were characterized by Fourier transform infrared (FTIR) spectroscopy, whereas scanning electron microscopy (SEM) described the morphologies of NPs. MTT and qRT-PCR assays were used to examine cell viability and the expression levels of various inflammatory cytokines. The cyclic voltammetry (CV) was employed to evaluate the redox potential of quercetin-Fe/quercetin-Fe complexes.
The material characterization results supported the loading of quercetin molecules on ZnO NPs. The CV and redox potential assays gave Fe-binding capability of quercetin at 0.15 mM and 0.3 mM of Fe(NO). Cytotoxicity assays using quercetin@ZnO NPs with human HaCaT cells showed no cytotoxic effects and help regain cell viability loss following UVA (150 kJ/m).
Quercetin@ZnO NPs showed that efficient quercetin release action is UV-controlled, and the released quercetin molecules have excellent antioxidant, anti-inflammatory, and iron sequestration potential. Quercetin@ZnO NPs have superior biocompatibility to provide UVA protection and medication at once for antiphotoaging therapeutics.
太阳紫外线辐射 A（UVA，320-400nm）是导致各种人类皮肤状况的重要风险因素，如皮肤过早衰老或光老化。这种情况因 UVA 介导的细胞铁蛋白中铁释放而加剧，影响到全球范围内的大量人群。
制备负载槲皮素的氧化锌纳米粒子（槲皮素@ZnO NPs），以研究其细胞铁螯合能力，以防止活性氧（ROS）的产生和 HaCaT 细胞的炎症反应。
通过均相沉淀法制备槲皮素@ZnO NPs，通过傅里叶变换红外（FTIR）光谱对其官能团进行表征，而扫描电子显微镜（SEM）则描述了 NPs 的形态。通过 MTT 和 qRT-PCR 测定法检测细胞活力和各种炎症细胞因子的表达水平。采用循环伏安法（CV）评估槲皮素-Fe/槲皮素-Fe 配合物的氧化还原电位。
材料特性研究结果支持了槲皮素分子在 ZnO NPs 上的负载。CV 和氧化还原电位测定结果表明，槲皮素在 0.15mM 和 0.3mM 的 Fe（NO）中具有 Fe 结合能力。用槲皮素@ZnO NPs 处理人 HaCaT 细胞的细胞毒性试验表明，没有细胞毒性作用，并有助于恢复 UVA（150kJ/m）照射后细胞活力的丧失。
槲皮素@ZnO NPs 表明，高效的槲皮素释放作用是受 UV 控制的，释放的槲皮素分子具有出色的抗氧化、抗炎和铁螯合潜力。与提供 UVA 保护和药物治疗同时进行的抗光老化治疗相比，槲皮素@ZnO NPs 具有更好的生物相容性。
