Rachitha Puttasiddaiah, Krupashree K, Kandikattu Hemanth Kumar, Nagaraj Geetha, Alahmadi Tahani Awad, Alharbi Sulaiman Ali, Shanmuganathan Rajasree, Brindhadevi Kathirvel, Raghavendra Vinay Basavegowda
P.G. Department of Biotechnology, Teresian College, Siddarthanagar, Mysore, 570011, India.
Department of Biochemistry, CSIR- Central Food Technological Research Institute, Mysuru, 570020, Karnataka, India.
Environ Res. 2023 Jun 1;226:115659. doi: 10.1016/j.envres.2023.115659. Epub 2023 Mar 9.
Allium sativum (A. sativum)is well known for its therapeutic and culinary uses. Because of their high medicinal properties, the clove extract was selected to synthesize cobalt-tellurium nanoparticles. The aim of the study was to evaluate the protective activity of the nanofabricated cobalt-tellurium using A. sativum (Co-Tel-As-NPs) against HO-induced oxidative damage in HaCaT cells. Synthesized Co-Tel-As-NPs were analyzed using UV-Visible spectroscopy, FT-IR, EDAX, XRD, DLS, and SEM. Various concentrations of Co-Tel-As-NPs were used as a pretreatment on HaCaT cells before HO was added. Then, the cell viability and mitochondrial damage were compared between pretreated and untreated control cells using an array of assays (MTT, LDH, DAPI, MMP, and TEM), and the intracellular ROS, NO, and antioxidant enzyme production were examined. In the present research, Co-Tel-As-NPs at different concentrations (0.5, 1.0, 2.0, and 4.0μg/mL) were tested for toxicity using HaCaT cells. Furthermore, the effect of HO on the viability of HaCaT cells was evaluated using the MTT assay for Co-Tel-As-NPs. Among those, Co-Tel-As-NPs at 4.0 μg/mL showed notable protection; with the same treatment, cell viability was discovered to be 91% and LDH leakage was also significantly decreased. Additionally, the measurement of mitochondrial membrane potential was significantly decreased by Co-Tel-As-NPs pretreatment against HO. The recovery of the condensed and fragmented nuclei brought about by the action of Co-Tel-As-NPs was identified using DAPI staining. TEM examination of the HaCaT cells revealed that the Co-Tel-As-NPs had a therapeutic effect against HO keratinocyte damage.
大蒜（Allium sativum）因其治疗和烹饪用途而广为人知。由于其具有较高的药用特性，因此选择丁香提取物来合成钴 - 碲纳米颗粒。本研究的目的是评估使用大蒜合成的纳米钴 - 碲（Co - Tel - As - NPs）对过氧化氢（HO）诱导的HaCaT细胞氧化损伤的保护活性。使用紫外可见光谱、傅里叶变换红外光谱、能谱分析、X射线衍射、动态光散射和扫描电子显微镜对合成的Co - Tel - As - NPs进行分析。在添加HO之前，将不同浓度的Co - Tel - As - NPs用作HaCaT细胞的预处理。然后，使用一系列检测方法（MTT、LDH、DAPI、线粒体膜电位检测和透射电子显微镜）比较预处理细胞和未处理对照细胞的细胞活力和线粒体损伤，并检测细胞内活性氧、一氧化氮和抗氧化酶的产生。在本研究中，使用HaCaT细胞测试了不同浓度（0.5、1.0、2.0和4.0μg/mL）的Co - Tel - As - NPs的毒性。此外，使用MTT检测法评估HO对Co - Tel - As - NPs处理的HaCaT细胞活力的影响。其中，4.0μg/mL的Co - Tel - As - NPs显示出显著的保护作用；经过相同处理后，发现细胞活力为91%，乳酸脱氢酶泄漏也显著减少。此外，Co - Tel - As - NPs预处理可显著降低HO引起的线粒体膜电位的降低。使用DAPI染色鉴定了Co - Tel - As - NPs作用导致的浓缩和碎片化细胞核的恢复情况。对HaCaT细胞的透射电子显微镜检查显示，Co - Tel - As - NPs对HO诱导的角质形成细胞损伤具有治疗作用。
