Roberto Vânia P, Surget Gwladys, Le Lann Klervi, Mira Sara, Tarasco Marco, Guérard Fabienne, Poupart Nathalie, Laizé Vincent, Stiger-Pouvreau Valérie, Cancela M Leonor
Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal.
University of Brest, CNRS, IRD, Ifremer, LEMAR, IUEM, Plouzané, France.
Front Nutr. 2021 Aug 18;8:719438. doi: 10.3389/fnut.2021.719438. eCollection 2021.
Osteoporosis is an aging-related disease and a worldwide health issue. Current therapeutics have failed to reduce the prevalence of osteoporosis in the human population, thus the discovery of compounds with bone anabolic properties that could be the basis of next generation drugs is a priority. Marine plants contain a wide range of bioactive compounds and the presence of osteoactive phytochemicals was investigated in two halophytes collected in Brittany (France): the invasive and the native . Two semi-purified fractions, prepared through liquid-liquid extraction, were assessed for phenolic and flavonoid contents, and for the presence of antioxidant, mineralogenic and osteogenic bioactivities. Ethyl acetate fraction (EAF) was rich in phenolic compounds and exhibited the highest antioxidant activity. While EAF only triggered a weak proliferative effect EAF potently induced extracellular matrix mineralization (7-fold at 250 μg/mL). A strong osteogenic effect was also observed using zebrafish operculum assay (2.5-fold at 10 μg/mL in 9-dpf larvae). Results indicate that polyphenol rich EAF of has both antioxidant and bone anabolic activities. As an invasive species, this marine plant may represent a sustainable source of molecules for therapeutic applications in bone disorders.
骨质疏松症是一种与衰老相关的疾病，也是一个全球性的健康问题。目前的治疗方法未能降低人类骨质疏松症的患病率，因此，发现具有骨合成代谢特性、可作为下一代药物基础的化合物是当务之急。海洋植物含有多种生物活性化合物，我们对在法国布列塔尼采集的两种盐生植物（入侵种和本地种）中的骨活性植物化学物质进行了研究。通过液-液萃取制备了两个半纯化级分，评估了它们的酚类和黄酮类含量，以及抗氧化、矿化和成骨生物活性。乙酸乙酯级分（EAF）富含酚类化合物，具有最高的抗氧化活性。虽然EAF仅引发微弱的增殖效应，但EAF能有效诱导细胞外基质矿化（在250μg/mL时为7倍）。使用斑马鱼鳃盖试验也观察到了强烈的成骨作用（在9日龄幼虫中，10μg/mL时为2.5倍）。结果表明，富含多酚的EAF具有抗氧化和成骨活性。作为一种入侵物种，这种海洋植物可能是治疗骨疾病的可持续分子来源。
