Department of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China.
Biomed Res Int. 2021 Aug 26;2021:5473959. doi: 10.1155/2021/5473959. eCollection 2021.
The hypercoagulable status, which forms a vicious cycle with hematogenous metastasis, is a common systemic alteration in cancers. As modeling is a key approach in research, a model which is suitable for studying how the hypercoagulable status promotes hematogenous metastasis in breast cancer is urgently needed.
Based on the tumor-bearing period (TBP) and postoperative incubation period (PIP), 4T1-breast cancer models were constructed to evaluate coagulation and tumor burden to generate multiple linear regression-based lung metastasis prediction formula. Platelets and 4T1 cells were cocultured for 30 min or 24 h to evaluate the early and late phases of their crosstalk, and then the physical characteristics (concentration and size) and procoagulant activity of the coculture supernatants were assayed.
The multiple linear regression model was constructed as log10 (photon number) = 0.147 TBP + 0.14 PIP + 3.303 (TBP ≤ 25 and PIP ≤ 17) to predict lung metastasis. Coculture of platelets and 4T1 cells contributed to the release of extracellular vesicles (EVs) and the development of the hypercoagulable status.
and hypercoagulable status models were developed to explore the mechanism of hypercoagulable status which is characterized by platelet activation and promotes hematogenous metastasis in breast cancer.
高凝状态与血行转移形成恶性循环，是癌症的一种常见全身改变。由于建模是研究的关键方法，因此迫切需要建立一种适合研究高凝状态如何促进乳腺癌血行转移的模型。
基于荷瘤期（TBP）和术后孵育期（PIP），构建了 4T1 乳腺癌模型，以评估凝血和肿瘤负担，从而生成基于多元线性回归的肺转移预测公式。血小板和 4T1 细胞共培养 30 分钟或 24 小时，以评估它们相互作用的早期和晚期阶段，然后测定共培养上清液的物理特性（浓度和大小）和促凝活性。
构建了多元线性回归模型，log10（光子数）=0.147 TBP+0.14 PIP+3.303（TBP≤25 且 PIP≤17），以预测肺转移。血小板和 4T1 细胞的共培养有助于细胞外囊泡（EVs）的释放和高凝状态的发展。
本研究建立了高凝状态模型，以探讨高凝状态的机制，该状态的特征是血小板激活并促进乳腺癌的血行转移。
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