Division of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Department of Pathology, Xiangyang Central Hospital, Xiangcheng District, Xiangyang, Hubei, China.
Biomed Res Int. 2021 Aug 24;2021:6529255. doi: 10.1155/2021/6529255. eCollection 2021.
MicroRNA-1-3p (miR-1-3p) exerts significant regulation in various tumor cells, but its molecular mechanisms in head and neck squamous cell carcinoma (HNSCC) are still ill defined. This study is aimed at detecting the expression of miR-1-3p in HNSCC and at determining its significant regulatory pathways.
Data were obtained from the Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), Oncomine, ArrayExpress, Sequence Read Archive (SRA) databases, and additional literature. Expression values of miR-1-3p in HNSCC were analyzed comprehensively. The R language software was employed to screen differentially expressed genes, and bioinformatics assessment was performed. One sequence dataset (HNSCC: = 484; noncancer: = 44) and 18 chip datasets (HNSCC: = 656; noncancer: = 199) were obtained.
The expression of miR-1-3p in HNSCC was visibly decreased in compare with noncancerous tissues. There were distinct differences in tumor state ( = 0.0417), pathological stage ( = 0.0058), and T stage ( = 0.0044). Comprehensive analysis of sequence and chip data also indicated that miR-1-3p was lowly expressed in HNSCC. The diagnostic performance of miR-1-3p in HNSCC is reflected in the sensitivity and specificity of the collection, etc. Bioinformatics analysis showed the possible biological process, cellular component, molecular function, and KEGG pathways of miR-1-3p in HNSCC. And was a possible target of miR-1-3p.
miR-1-3p's low expression may facilitate tumorigenesis and evolution in HNSCC through signaling pathways. may be a key gene in targeting pathways but still needs verification through in vitro experiments.
微小 RNA-1-3p（miR-1-3p）在各种肿瘤细胞中发挥着重要的调控作用，但它在头颈部鳞状细胞癌（HNSCC）中的分子机制仍未明确。本研究旨在检测 miR-1-3p 在 HNSCC 中的表达情况，并确定其重要的调控途径。
从癌症基因组图谱（TCGA）、基因表达综合数据库（GEO）、Oncomine、ArrayExpress、序列读取档案（SRA）数据库以及其他文献中获取数据。综合分析 miR-1-3p 在 HNSCC 中的表达情况。使用 R 语言软件筛选差异表达基因，并进行生物信息学评估。共获得 1 个序列数据集（HNSCC：=484；非癌组织：=44）和 18 个芯片数据集（HNSCC：=656；非癌组织：=199）。
与非癌组织相比，HNSCC 中 miR-1-3p 的表达明显降低。肿瘤状态（=0.0417）、病理分期（=0.0058）和 T 分期（=0.0044）差异显著。序列和芯片数据的综合分析也表明 miR-1-3p 在 HNSCC 中低表达。miR-1-3p 在 HNSCC 中的诊断性能反映在集合的灵敏度和特异性等方面。生物信息学分析显示 miR-1-3p 在 HNSCC 中的可能生物学过程、细胞成分、分子功能和 KEGG 途径。 可能是 miR-1-3p 的一个靶基因。
miR-1-3p 的低表达可能通过信号通路促进 HNSCC 的发生和演进。 可能是靶向通路的关键基因，但仍需要通过体外实验进行验证。
