Meng Li, Cai Qing, Zhang Huaijian, Gao Zhiqin, Yang Lianjuan
Department of Dermatological Mycology, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443, P.R. China.
Biomed Rep. 2024 Nov 28;22(2):20. doi: 10.3892/br.2024.1898. eCollection 2025 Feb.
Non-coding small molecule RNAs are associated with a variety of diseases, including infectious diseases. However, small RNA-related studies in onychomycosis have not been reported. The aim of the present study was to conduct an initial investigation of small RNA in onychomycosis. The present study collected a total of 33 affected nail samples from patients with onychomycosis and 18 normal nail samples from healthy people. Through RNA sequencing, 37 differentially expressed microRNAs (miRNAs or miRs), including 15 upregulated and 22 downregulated miRNAs, were identified in 3 patients with onychomycosis compared with 3 healthy controls. Moreover, three differentially expressed miRNAs were analyzed for further verification by RT-qPCR in other 30 affected nail and 15 healthy nail samples. Among the three verified miRNAs, a significant difference between the downregulated hsa-miR-1-3p and hsa-miR-361-3p was observed (P<0.05). A total of 14,511 target genes of 37 differentially expressed miRNAs were predicted by the miRanda and RNAhybrid databases, while the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analysis showed that these target genes were enriched in multiple signaling pathways. The present study indicated that hsa-miR-1-3p and hsa-miR-361-3p may be potential biomarkers for onychomycosis. Furthermore, the findings of the present study can be used in future research on RNA in onychomycosis.
非编码小分子RNA与多种疾病相关，包括传染病。然而，关于甲癣的小RNA相关研究尚未见报道。本研究的目的是对甲癣中的小RNA进行初步调查。本研究共收集了33例甲癣患者的患甲样本和18例健康人的正常指甲样本。通过RNA测序，与3名健康对照相比，在3例甲癣患者中鉴定出37种差异表达的微小RNA（miRNA或miR），其中15种上调，22种下调。此外，对另外30例患甲样本和15例健康指甲样本中的三种差异表达miRNA进行了RT-qPCR进一步验证。在三种经验证的miRNA中，下调的hsa-miR-1-3p和hsa-miR-361-3p之间观察到显著差异（P<0.05）。通过miRanda和RNAhybrid数据库预测了37种差异表达miRNA的总共14511个靶基因，而京都基因与基因组百科全书和基因本体分析表明这些靶基因在多个信号通路中富集。本研究表明，hsa-miR-1-3p和hsa-miR-361-3p可能是甲癣的潜在生物标志物。此外，本研究结果可用于未来甲癣中RNA的研究。
