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清醒兔 1 型糖尿病合并心肌缺血/再灌注损伤的临床前模型——雷帕霉素的心脏保护作用。

Preclinical model of type 1 diabetes and myocardial ischemia/reperfusion injury in conscious rabbits-demonstration of cardioprotection with rapamycin.

机构信息

Division of Cardiology, Pauley Heart Center, Virginia Commonwealth University, Richmond, VA 23298, USA.

出版信息

STAR Protoc. 2021 Aug 24;2(3):100772. doi: 10.1016/j.xpro.2021.100772. eCollection 2021 Sep 17.

DOI:10.1016/j.xpro.2021.100772
PMID:34485939
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8403684/
Abstract

We developed a preclinical model of myocardial ischemia/reperfusion (I/R) injury in conscious diabetic rabbits to identify an early pharmacological intervention for patients with diabetes and acute myocardial infarction (AMI). Here, we describe a reproducible protocol for induction of diabetes with subsequent manifestation of myocardial I/R injury in conscious rabbits to mimic the real-life scenario observed in clinical settings. Further, we demonstrate the efficacy of rapamycin at the onset of reperfusion to limit the adverse effect of AMI. For complete details on the use and execution of this protocol, please refer to Samidurai et al. (2020).

摘要

我们建立了一种清醒糖尿病兔心肌缺血/再灌注（I/R）损伤的临床前模型，旨在寻找一种针对糖尿病合并急性心肌梗死（AMI）患者的早期药物干预手段。在此，我们描述了一种在清醒兔中诱导糖尿病并随后产生心肌 I/R 损伤的重现性方案，以模拟临床环境中观察到的真实情况。此外，我们还证明了雷帕霉素在再灌注开始时的疗效，以限制 AMI 的不良影响。如需详细了解本方案的使用和实施，请参考 Samidurai 等人（2020 年）的研究。

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Cardiovasc Res. 2020 Nov 1;116(13):2103-2115. doi: 10.1093/cvr/cvz315.
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Sacubitril/Valsartan Averts Adverse Post-Infarction Ventricular Remodeling and Preserves Systolic Function in Rabbits.
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