Virginia Institute of Marine Science, William & Mary, Gloucester Point, Virginia, USA.
J Appl Microbiol. 2022 Feb;132(2):760-771. doi: 10.1111/jam.15287. Epub 2021 Sep 17.
To investigate the relationships between individual health status of oysters, particularly with regard to parasitic infection, and variability in abundance of human-pathogenic Vibrio species.
Aquacultured eastern oysters, Crassostrea virginica, were analysed individually for infection by the protozoan parasite Perkinsus marinus through quantitative PCR, and total Vibrio vulnificus and total and pathogenic Vibrio parahaemolyticus abundance was assessed using a most probable number (MPN)-qPCR approach. Additionally, perspective on general oyster health and other parasitic infections was obtained through histopathology. Perkinsus marinus infection and human-pathogenic Vibrio species levels were not correlated, but through histology, analyses revealed that oysters infected by Haplosporidium nelsoni harboured more V. vulnificus.
The highly prevalent parasite P. marinus had little influence on human-pathogenic Vibrio species levels in eastern oysters, but the less prevalent parasite, H. nelsoni, may influence V. vulnificus levels, highlighting the potential nuances of within-oyster dynamics of Vibrio species.
Human-pathogenic bacteria continue to be a concern to the oyster industry and causes for individual oyster variation in bacterial levels remain unknown. The major oyster pathogen P. marinus does not appear to affect levels of these bacteria within oysters, suggesting that other factors may influence Vibrio spp. levels in oysters.
研究牡蛎个体健康状况（特别是寄生虫感染）与人类病原体弧菌物种丰度变化之间的关系。
通过定量 PCR 分析养殖东方牡蛎（Crassostrea virginica）中原生动物寄生虫 Perkinsus marinus 的感染情况，并使用最可能数（MPN）-qPCR 方法评估总弧菌 vulnificus 和总致病性弧菌 parahaemolyticus 的丰度。此外，通过组织病理学获得了对牡蛎一般健康状况和其他寄生虫感染的看法。 Perkinsus marinus 感染和人类病原体弧菌种类水平没有相关性，但通过组织学分析表明，感染 Haplosporidium nelsoni 的牡蛎携带更多的 V. vulnificus。
高度流行的寄生虫 P. marinus 对东方牡蛎中人类病原体弧菌种类水平影响不大，但不太流行的寄生虫 H. nelsoni 可能会影响 V. vulnificus 水平，突出了弧菌种类在牡蛎体内动态的潜在细微差别。
人类病原体细菌仍然是牡蛎产业关注的问题，而个体牡蛎细菌水平变化的原因仍不清楚。主要的牡蛎病原体 P. marinus 似乎不会影响这些细菌在牡蛎体内的水平，这表明其他因素可能会影响牡蛎中弧菌的水平。
