Gharaghani Maral, Rezaei-Matehkolaei Ali, Hardani Amir Kamal, Zarei Mahmoudabadi Ali
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Pediatrics, School of Medicine, Abuzar Children's Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Microb Pathog. 2021 Nov;160:105173. doi: 10.1016/j.micpath.2021.105173. Epub 2021 Sep 4.
The presence of Candida species in urine may be due to colonization of this species in the bladder, urinary catheter, and perineum. Candida albicans has been the most commonly isolated from urine samples in patients with candiduria. Several virulence factors include adhesion to host cells, secreted extracellular enzymes, phenotype switching, and biofilm formation are contributing to the pathogenicity of C. albicans. ABC genotyping is the method based on the determination of 25s rDNA and C. albicans is divided into four genotypes include A, B, C, and E. We aimed to identify Candida species from pediatrics and evaluate extracellular enzyme activities, phenotype switching, biofilm formation, and genotyping in isolates. Urine samples collected, cultured, and yielded yeasts were identified. Phenotype switching, biofilm formation, enzymatic patterns, and genotyping of 50 isolates of C. albicans were evaluated. The Genotyping pattern was compared with extracellular enzymes, biofilm formation, and phenotype switching pattern. 16.2% of urine cultures were positive for the different Candida species. The most common species was C. albicans, followed by C. glabrata. Out of 50 isolates of C. albicans, 72% and 28% isolates were recognized as genotypes A and C. All isolates were produced extracellular enzymes and biofilm formation. In conclusion, candiduria with high colony counts is still a challenge in Iranian pediatrics. Genotype A was the predominant genotype among C. albicans strains. There is a statistical difference between esterase and genotypes of C and A C. albicans.
尿液中念珠菌属的存在可能是由于该菌在膀胱、导尿管和会阴部定植所致。白色念珠菌是念珠菌尿患者尿液样本中最常分离出的菌种。几种毒力因子，包括对宿主细胞的黏附、分泌的细胞外酶、表型转换和生物膜形成，都促成了白色念珠菌的致病性。ABC基因分型是基于对25s rDNA的测定的方法，白色念珠菌分为四种基因型，即A、B、C和E。我们旨在从儿科患者中鉴定念珠菌属菌种，并评估分离株的细胞外酶活性、表型转换、生物膜形成和基因分型。对收集、培养并产生酵母的尿液样本进行鉴定。评估了50株白色念珠菌分离株的表型转换、生物膜形成、酶谱和基因分型。将基因分型模式与细胞外酶、生物膜形成和表型转换模式进行比较。16.2%的尿液培养物对不同的念珠菌属菌种呈阳性。最常见的菌种是白色念珠菌，其次是光滑念珠菌。在50株白色念珠菌分离株中，72%和28%的分离株被鉴定为基因型A和C。所有分离株均产生细胞外酶并形成生物膜。总之，高菌落计数的念珠菌尿在伊朗儿科中仍然是一个挑战。基因型A是白色念珠菌菌株中的主要基因型。白色念珠菌C型和A型的酯酶与基因型之间存在统计学差异。
