Laboratório de Micologia Médica e Molecular, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
BMC Oral Health. 2014 Mar 15;14:20. doi: 10.1186/1472-6831-14-20.
Candida albicans is a diploid yeast that in some circumstances may cause oral or oropharyngeal infections. This investigation aimed to study the prevalence of Candida spp. and to analyze the ABC genotypes of 76 clinical isolates of C. albicans obtained from the oral cavity of kidney transplant patients from two distinct geographic regions of Brazil.
We typed 48 strains with ABC genotyping and Microsatelitte using primer M13 and tested three virulence factors in vitro: phospholipase activity, morphogenesis and the ability to evade from polymorphonuclear neutrophils phagocytosis.
C. albicans was the most prevalent species (86.4%), followed by C. tropicalis (4.5%). C. albicans genotype A was the most prevalent (58 isolates; 76.4%), followed by genotype C (15 isolates; 19.7%) and genotype B (3 isolates; 3.9%). When Microsatellite technique with primer M13 was applied, 80% of the isolates from the South were placed within the same cluster. The majority of Genotype C strains were grouped together within two different clusters. Genotype C was considered more resistant to PMNs attack than genotypes A and B. Strains isolated from the South of Brazil showed also better ability to combat PMNs phagocytosis.
We found a high rate of C. albicans genotype C strains isolated from the oral cavity of this group of patients. This study characterized oral C. albicans strains isolated from kidney transplant recipients and will contribute to a better understanding of the pathogenesis of oral candidiasis.
白色念珠菌是一种二倍体酵母，在某些情况下可能引起口腔或口咽感染。本研究旨在调查巴西两个不同地区的肾移植患者口腔分离的念珠菌属（Candida spp.）的流行情况，并分析 76 株白色念珠菌的 ABC 基因型。
我们对 48 株进行 ABC 基因分型和微卫星分型，使用引物 M13 进行微卫星分型，并在体外测试三种毒力因子：磷脂酶活性、形态发生和逃避多形核白细胞吞噬作用的能力。
白色念珠菌最为常见（86.4%），其次是热带念珠菌（4.5%）。A 基因型最为常见（58 株；76.4%），其次是 C 基因型（15 株；19.7%）和 B 基因型（3 株；3.9%）。当使用引物 M13 的微卫星技术时，来自南部的 80%的分离株被归入同一簇。大多数 C 基因型菌株聚集在两个不同的簇内。与 A 基因型和 B 基因型相比，C 基因型被认为对 PMNs 的攻击具有更强的抗性。来自巴西南部的菌株也显示出更好的逃避 PMNs 吞噬的能力。
我们发现，从这群患者口腔分离的白色念珠菌 C 基因型菌株的比例很高。本研究对肾移植受者口腔分离的白色念珠菌进行了特征分析，将有助于更好地了解口腔念珠菌病的发病机制。
