Bioengineering and Sustainable Processes Research Group, Department of Chemical Engineering, University of Vigo, Vigo, 36310, Spain; Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, Giza, 12622, Egypt.
Bioengineering and Sustainable Processes Research Group, Department of Chemical Engineering, University of Vigo, Vigo, 36310, Spain.
Enzyme Microb Technol. 2021 Oct;150:109865. doi: 10.1016/j.enzmictec.2021.109865. Epub 2021 Jul 1.
In this study, we cross-linked aminated Thermothelomyces thermophilus laccase onto Immobead 150P epoxy carrier, and achieved an immobilization yield of 99.84 %. The optimum temperature and pH values for the oxidation of ABTS by laccase were determined to be 70 °C and pH 3.0. After 6 h at 50 °C, laccase activity was diminished by about 13 % in the free form and 28 %, in the immobilized form. K values for both free and cross-linked laccase were 0.051 and 0.567 mM, whereas V values were 2.027 and 0.854 μmol. min, respectively. The immobilized laccase was able to preserve its full activity for 6 weeks, retaining approximately 95 % and 78 % of its initial activity after 8 and 20 weeks, respectively. The contact angles were two-fold higher when the laccase enzyme was occupied in the biografting reaction, revealing that the hydrophobic compound bonded stably onto beechwood samples.
在这项研究中，我们将氨基化嗜热真菌漆酶交联到 Immobead 150P 环氧载体上，实现了 99.84%的固定化产率。确定漆酶氧化 ABTS 的最佳温度和 pH 值分别为 70°C 和 pH 3.0。在 50°C 下 6 小时后，游离形式的漆酶活性降低了约 13%，固定化形式的漆酶活性降低了 28%。游离和交联漆酶的 K 值分别为 0.051 和 0.567 mM，而 V 值分别为 2.027 和 0.854 μmol·min。固定化漆酶能够在 6 周内保持其全部活性，在 8 周和 20 周后分别保留初始活性的约 95%和 78%。当漆酶酶参与生物接枝反应时，接触角增加了一倍，表明疏水性化合物稳定地结合到山毛榉样本上。
