Othman Abdelmageed M, Flaifil Angelina G
Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt.
Faculty of Biotechnology, German International University, Regional Ring Road, East Cairo, New Administrative Capital, Cairo, Egypt.
Sci Rep. 2025 Jan 3;15(1):735. doi: 10.1038/s41598-024-82310-0.
In the current study, calcium alginate was used as a carrier for Agaricus bisporus CU13 laccase immobilization, with an immobilization yield of the entrapped laccase of 91.95%. Free and immobilized enzymes showed their best enzyme activity at 60 °C as an optimum temperature. Free laccase was nearly completely inactivated at high temperatures (70 and 80 °C C) after 30 min, whereas the immobilized form retained around 60 and 40% of its activity after 30 min at the same temperatures. The metal ion of MgSO showed the best impact on the Agaricus bisporus laccase activity with a relative activity of 113.1 and 106.8% at concentrations of 10 mM and 2.5 mM, respectively. The best Cibacron D-Blue SGL dye degradation by laccase enzyme was obtained at pH 6.0, 0.354 U of laccase, and 100 mg/L of dye using hydroxybenzotriazole (HBT; 1 mM) as a mediator. Optimization ramps obtained from the central composite design (CCD) approach indicate that the rate at which the laccase enzyme decolorizes dye has increased significantly when the concentrations of the enzyme, and HBT increased, whereas dye concentration decreased. Finally, the immobilized enzyme was found to be efficient in decolorizing Cibacron D-Blue SGL dye in the presence of HBT as a mediator for different cycles.
在当前研究中，海藻酸钙被用作双孢蘑菇CU13漆酶固定化的载体，包埋漆酶的固定化产率为91.95%。游离酶和固定化酶在60℃时表现出最佳酶活性，为最适温度。游离漆酶在高温（70℃和80℃）下30分钟后几乎完全失活，而固定化形式在相同温度下30分钟后仍保留约60%和40%的活性。MgSO的金属离子对双孢蘑菇漆酶活性的影响最佳，在浓度分别为10 mM和2.5 mM时，相对活性分别为113.1%和106.8%。使用羟基苯并三唑（HBT；1 mM）作为介质，在pH 6.0、漆酶0.354 U和染料100 mg/L的条件下，漆酶对汽巴克隆D-蓝SGL染料的降解效果最佳。从中心复合设计（CCD）方法获得的优化曲线表明，当酶和HBT的浓度增加而染料浓度降低时，漆酶使染料脱色的速率显著提高。最后，发现固定化酶在存在HBT作为介质的情况下，对不同循环的汽巴克隆D-蓝SGL染料进行脱色是有效的。
