Miya Ken, Keino-Masu Kazuko, Okada Takuya, Kobayashi Kenta, Masu Masayuki
Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
Department of Molecular Neurobiology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Front Neuroanat. 2021 Aug 20;15:726718. doi: 10.3389/fnana.2021.726718. eCollection 2021.
The heparan sulfate 6--endosulfatases, Sulfatase 1 (Sulf1), and Sulfatase 2 (Sulf2), are extracellular enzymes that regulate cellular signaling by removing 6--sulfate from the heparan sulfate chain. Although previous studies have revealed that are essential for normal development, their functions in the adult brain remain largely unknown. To gain insight into their neural functions, we used hybridization to systematically examine mRNA expression in the adult mouse brain. and mRNAs showed distinct expression patterns, which is in contrast to their overlapping expression in the embryonic brain. In addition, we found that was distinctly expressed in the nucleus accumbens shell, the posterior tail of the striatum, layer 6 of the cerebral cortex, and the paraventricular nucleus of the thalamus, all of which are target areas of dopaminergic projections. Using double-labeling techniques, we showed that -expressing cells in the above regions coincided with cells expressing the dopamine D1 and/or D2 receptor. These findings implicate possible roles of in modulation of dopaminergic transmission and dopamine-mediated behaviors.
硫酸乙酰肝素6-O-硫酸酯酶、硫酸酯酶1（Sulf1）和硫酸酯酶2（Sulf2）是细胞外酶，通过从硫酸乙酰肝素链上去除6-O-硫酸酯来调节细胞信号传导。尽管先前的研究表明它们对正常发育至关重要，但其在成体大脑中的功能仍 largely未知。为了深入了解它们的神经功能，我们使用杂交技术系统地检测了成年小鼠大脑中Sulf1和Sulf2的mRNA表达。Sulf1和Sulf2的mRNA呈现出不同的表达模式，这与其在胚胎大脑中的重叠表达形成对比。此外，我们发现Sulf1在伏隔核壳、纹状体后尾、大脑皮层第6层和丘脑室旁核中均有明显表达，所有这些都是多巴胺能投射的靶区。使用双标记技术，我们表明上述区域中表达Sulf1的细胞与表达多巴胺D1和/或D2受体的细胞一致。这些发现提示Sulf1在调节多巴胺能传递和多巴胺介导的行为中可能发挥作用。
