Xiang Mei, Zhang Hong-Zhi, Jing Xiao-Yu, Wang Meng-Qing, Mao Jian-Jun, Li Yu-Yan, Zang Lian-Sheng, Zhang Li-Sheng
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Institute of Biological Control, Jilin Agricultural University, Changchun, China.
Front Physiol. 2021 Aug 19;12:706032. doi: 10.3389/fphys.2021.706032. eCollection 2021.
The ladybird L., a predatory insect, serves as an excellent biological control agent against common agricultural pests. It undergoes a diapause phenomenon, during which a large amount of fat accumulates in the abdomen. A comprehensive analysis of this lipid accumulation can reveal the molecular mechanisms underlying diapause regulation, which can be exploited to improve the shipping and transport of the insect for agricultural applications. In this study, we compared the transcriptome of during non-diapause, diapause, and post-diapause and screened four key genes related to lipid metabolism. The cDNA of these four relevant enzymes, acetyl-CoA carboxylase (ACC), long-chain fatty acid-CoA ligase (ACSL), elongase of very-long-chain fatty acids (ELO), and very-long-chain 3-oxoacyl-CoA reductase (KAR), were cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. Their expression profiles were analyzed during the preparation and maintenance phases of diapause and the post-diapause phase. The functions of these four key enzymes in diapause were further verified using RNA interference. All four genes were most closely related to the homeotic gene from . The expression profiles of these four genes were significantly affected under diapause-inducing conditions; their expression level was the highest in the diapause preparation phase, and it gradually decreased with the diapause induction time. RNA interference showed that the target genes play important roles in fat storage during early diapause, and the decrease in their expression leads to a decrease in lipid content in . These results indicate an important role of , , , and in lipid accumulation. Our findings could help elucidate the production and accumulation of lipids by insects during the preparation for diapause and improve biological control.
瓢虫,一种捕食性昆虫,是防治常见农业害虫的优秀生物防治剂。它会经历滞育现象,在此期间腹部会积累大量脂肪。对这种脂质积累进行全面分析可以揭示滞育调节的分子机制,这可用于改善该昆虫在农业应用中的运输。在本研究中,我们比较了非滞育、滞育和滞育后阶段的转录组,并筛选出四个与脂质代谢相关的关键基因。使用逆转录 - 聚合酶链反应和cDNA末端快速扩增技术克隆了这四种相关酶(乙酰辅酶A羧化酶(ACC)、长链脂肪酸辅酶A连接酶(ACSL)、超长链脂肪酸延长酶(ELO)和超长链3 - 氧代酰基辅酶A还原酶(KAR))的cDNA。分析了它们在滞育准备和维持阶段以及滞育后阶段的表达谱。使用RNA干扰进一步验证了这四种关键酶在滞育中的功能。所有四个基因与来自[具体物种]的同源异型基因关系最为密切。在滞育诱导条件下,这四个基因的表达谱受到显著影响;它们的表达水平在滞育准备阶段最高,并随着滞育诱导时间逐渐降低。RNA干扰表明,目标基因在滞育早期的脂肪储存中起重要作用,其表达的降低导致[具体昆虫名称]脂质含量下降。这些结果表明ACC、ACSL、ELO和KAR在脂质积累中起重要作用。我们的研究结果有助于阐明昆虫在滞育准备期间脂质的产生和积累,并改善生物防治。