Chen Yan-Rong, Jiang Tao, Zhu Juan, Xie Yu-Chen, Tan Zhi-Cheng, Chen Yan-Hua, Tang Shun-Ming, Hao Bi-Fang, Wang Sheng-Peng, Huang Jin-Shan, Shen Xing-Jia
Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China; Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China; Huangshan University, Huangshan 245041, China.
Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China; Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2017 Dec;24:68-78. doi: 10.1016/j.cbd.2017.07.003. Epub 2017 Aug 2.
In the bivoltine strain of the silkworm, Bombyx mori, embryonic diapause is induced transgenerationally as a maternal effect. Progeny diapause ability is determined by the environmental condition such as temperature and lightness that mothers experience during their own embryonic development. Diapause preparation is a crucial phase of this process; diapause-destined individuals undergo a series of preparatory events before the entry into developmental arrest. However, the molecular regulatory mechanisms of diapause preparation have largely remained unknown. In the present study, we sequenced the transcriptome of bivoltine silkworm Qiufeng's ovaries resulted in laying of diapause destined or non-diapause eggs, using high-throughput RNA-Seq technology. Differential expression analyses identified 183 genes with higher expression, and 106 with lower expression under diapause-inducing conditions. GO and KEGG analysis revealed that the enrichment of several functional terms related to peroxisome, glycerolipid metabolism, steroid biosynthesis, longevity regulating pathway - multiple species, three signaling transductions, insect hormone biosynthesis, and cytoskeleton components. We conducted a detailed comparison of transcript profile data of ovaries from diapause-inducing and non-diapause conditions, the results imply up-regulation of peroxisomal metabolism, triacylglycerides accumulation, cryoprotectant production, and ecdysteroid biosynthesis in diapause-inducing group. Differential expression of genes related to actin cytoskeleton implies the occurrence of shifts in cellular structure and composition between diapause-inducing and non-diapause-inducing groups. The Hippo and FOXO signaling pathways may play an important role in preparing for entering diapause. This study provides an insight into the molecular events of insect diapause, in particular for the preparatory phase.
在二化性家蚕品系中，胚胎滞育作为一种母体效应跨代诱导产生。子代的滞育能力由母体在自身胚胎发育过程中所经历的温度和光照等环境条件决定。滞育准备是这一过程的关键阶段；注定进入滞育的个体在进入发育停滞之前会经历一系列准备事件。然而，滞育准备的分子调控机制在很大程度上仍不清楚。在本研究中，我们利用高通量RNA测序技术，对二化性家蚕秋丰产下滞育卵或非滞育卵后的卵巢转录组进行了测序。差异表达分析确定了在滞育诱导条件下183个高表达基因和106个低表达基因。基因本体（GO）和京都基因与基因组百科全书（KEGG）分析表明，与过氧化物酶体、甘油脂质代谢、类固醇生物合成、多物种寿命调节途径、三种信号转导、昆虫激素生物合成以及细胞骨架成分相关的几个功能术语显著富集。我们对滞育诱导和非滞育条件下卵巢的转录谱数据进行了详细比较，结果表明滞育诱导组中过氧化物酶体代谢、三酰甘油积累、抗冻剂产生和蜕皮甾类生物合成上调。与肌动蛋白细胞骨架相关基因的差异表达表明滞育诱导组和非滞育诱导组之间细胞结构和组成发生了变化。Hippo和FOXO信号通路可能在进入滞育的准备过程中起重要作用。本研究深入了解了昆虫滞育的分子事件，特别是准备阶段。
