Zhang Maosen, He Weiwei, Li Yuyan, Chen Junjie, Teets Nicholas M, Zhang Lisheng
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Natural Enemy Insects, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
Department of Entomology, University of Kentucky, Lexington, KY 40546, USA.
iScience. 2025 Jan 6;28(3):111761. doi: 10.1016/j.isci.2025.111761. eCollection 2025 Mar 21.
Diapause enables insects to survive unfavorable conditions through metabolic and developmental adjustments. We investigated metabolic regulation during reproductive diapause in the predatory stinkbug using transcriptomic and metabolomic analyses. Our study revealed 9,254 differentially expressed genes and 493 significantly changed metabolites across diapause stages. Key metabolic pathways including glutathione metabolism, TCA cycle, glycolysis, and lipid metabolism underwent substantial reorganization. The pre-diapause phase showed increased energy consumption and lipid accumulation, while the maintenance phase exhibited restructuring of amino acid and glucose metabolism. We identified stage-specific metabolic signatures and potential regulatory mechanisms, including the roles of glutathione metabolism in redox regulation and insulin signaling in diapause control. This comprehensive characterization of metabolic reprogramming during diapause provides insights for improving biocontrol agent production and storage strategies.
滞育使昆虫能够通过代谢和发育调整来度过不利条件。我们使用转录组学和代谢组学分析方法，研究了捕食性椿象生殖滞育期间的代谢调控。我们的研究揭示了在滞育各阶段有9254个差异表达基因和493种显著变化的代谢物。包括谷胱甘肽代谢、三羧酸循环、糖酵解和脂质代谢在内的关键代谢途径发生了重大重组。滞育前期显示出能量消耗增加和脂质积累，而维持期则表现出氨基酸和葡萄糖代谢的重构。我们确定了阶段特异性的代谢特征和潜在的调控机制，包括谷胱甘肽代谢在氧化还原调节中的作用以及胰岛素信号在滞育控制中的作用。对滞育期间代谢重编程的这种全面表征为改进生物防治剂的生产和储存策略提供了见解。
