Immunogenetics Laboratory, Molecular Biology Department, Medicine School in São José do Rio Preto, São José do Rio Preto, Brazil.
Department of Cardiology and Cardiovascular Surgery, Medicine School in São José do Rio Preto, São José do Rio Preto, Brazil.
Front Immunol. 2021 Aug 13;12:714766. doi: 10.3389/fimmu.2021.714766. eCollection 2021.
Tissue damage observed in the clinical forms of chronic symptomatic Chagas disease seems to have a close relationship with the intensity of the inflammatory process. The objective of this study was to investigate whether the () and () polymorphisms are associated with the cardiac and digestive clinical forms of chronic Chagas disease. Possible influence of these genes polymorphisms on the left ventricular systolic dysfunction (LVSD) in patients with chronic Chagas heart disease was also evaluated. This study enrolled 185 patients with positive serology for classified according to the clinical form of the disease: cardiac (n=107) and digestive (n=78). Subsequently, patients with the cardiac form of the disease were sub-classified as with LVSD (n=52) and without LVSD (n=55). A control group was formed of 110 healthy individuals. Genotyping was performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP). Statistical analyzes were carried out using the Chi-square test and odds ratio with 95% confidence interval was also calculated to evaluate the risk association. MICA-129 allele with high affinity for the NKG2D receptor was associated to the LVSD in patients with CCHD. The haplotype and the KIR2DS2/KIR2DL2/KIR2DL3/C1 combination were associated to the digestive clinical form of the disease. Our data showed that the and polymorphisms may exert a role in the LVSD of cardiac patients, and in digestive form of Chagas disease.
在慢性有症状的恰加斯病的临床形式中观察到的组织损伤似乎与炎症过程的强度密切相关。本研究的目的是研究（）和（）多态性是否与慢性恰加斯病的心脏和消化临床形式相关。还评估了这些基因多态性对慢性恰加斯心脏病患者左心室收缩功能障碍（LVSD）的可能影响。本研究纳入了 185 名血清学阳性的患者，根据疾病的临床形式进行分类：心脏（n=107）和消化（n=78）。随后，将患有心脏形式的疾病的患者进一步分为 LVSD（n=52）和无 LVSD（n=55）。对照组由 110 名健康个体组成。通过聚合酶链反应-序列特异性寡核苷酸探针（PCR-SSOP）进行基因分型。使用卡方检验进行统计分析，并计算了优势比及其 95%置信区间，以评估风险关联。对于 NKG2D 受体具有高亲和力的 MICA-129 等位基因与 CCHD 患者的 LVSD 相关。（）和（）单倍型与疾病的消化临床形式相关。我们的数据表明，（）和（）多态性可能在心脏患者的 LVSD 中发挥作用，并且在恰加斯病的消化形式中发挥作用。
