Molecular Detection of Insecticide Resistance Mutations in from Sierra Leone Using Multiplex SNaPshot and Sequencing.

Vector control interventions including long-lasting insecticidal nets and indoor residual spraying are important for malaria control and elimination. And effectiveness of these interventions depends entirely on the high level of susceptibility of malaria vectors to insecticides. However, the insecticide resistance in majority of mosquito vector species across African countries is a serious threat to the success of vector control efforts with the extensive use of insecticides, while no data on insecticide resistance was reported from Sierra Leone in the past decade. In the present study, the polymerase chain reaction was applied for the identification of species of 757 dry adult female mosquitoes reared from larvae collected from four districts in Sierra Leone during May and June 2018. And the mutations of , , genes in were detected using SNaPshot and sequencing. As a result, one sample from Western Area Rural district belonged to , and 748 were identified. Furthermore, the mutations, west mutations and mutation were found. The overall frequency was 35.7%, 0.3%, 97.6% and 4.5% in A296G , A296S , and , respectively. The frequencies of A296G mutation ( < 0.001), mutation ( = 0.001) and mutation ( < 0.001) were unevenly distributed in four districts, respectively, while no statistical significance was found in A296S mutation ( = 0.868). In addition, multiple resistance patterns were also found. In conclusion, multiple mutations involved in insecticide resistance in populations in Sierra Leone were detected in the , A296G and alleles in the present study. It is necessary to monitor vector susceptibility levels to insecticides used in this country, and update the insecticide resistance monitoring and management strategy.