Department of Clinical Laboratory, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, China.
Microbiological Laboratory, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
Front Cell Infect Microbiol. 2021 Aug 19;11:727811. doi: 10.3389/fcimb.2021.727811. eCollection 2021.
This study aims to analyze the molecular epidemiology, resistance, and pathogenicity of subsp. isolated from children.
Whole genome sequencing was carried out, and molecular serotypes, sequence types, resistance genes, and virulence genes of subsp. isolates were analyzed. Antimicrobial susceptibility test was determined by commercialized microdilution method.
A total of three isolates of subsp. were isolated during 2015 to 2020. The molecular serotypes of the three strains were 61:c:z35, 61:l,v:1,5,7:[z57], and 65:k:z, respectively, and the sequence types were ST1845, ST233, and ST1263. All the three isolates were susceptible to ceftriaxone, ceftazidime, cefepime, amoxycillin/clavulanic acid, piperacillin/tazobactam, ertapenem, imipenem, levofloxacin, and trimethoprim/sulfamethoxazole. No other resistant gene was detected except . There were no resistant plasmids detected in all the three isolates. A total of 76 genes were present in all isolates, containing 49 genes of Type III Secretion System (T3SS) mediated by SPI-1and SPI-2, 13 genes of adherence (type 1 fimbriae, Agf, and MisL-related genes), 11 genes of iron uptake (Yersiniabactin), two genes of magnesium uptake, and one gene of typhoid toxin().
The serotypes and sequence types of subsp. isolates were rarely reported in children; all the subsp. isolates were susceptible to detected antibiotics; T3SS, adherence, iron uptake, magnesium uptake, and typhoid toxin were responsible for pathogenicity of the subsp. isolates in children.
本研究旨在分析儿童分离的 血清型、耐药性和致病性。
进行全基因组测序，分析 血清型、序列型、耐药基因和毒力基因。采用商业化微量稀释法进行药敏试验。
2015 年至 2020 年共分离出 3 株 血清型 。3 株菌的分子血清型分别为 61:c:z35、61:l,v:1,5,7:[z57]和 65:k:z，序列型分别为 ST1845、ST233 和 ST1263。三株菌均对头孢曲松、头孢他啶、头孢吡肟、阿莫西林/克拉维酸、哌拉西林/他唑巴坦、厄他培南、亚胺培南、左氧氟沙星和复方磺胺甲噁唑敏感。除 外，未检出其他耐药基因。三株菌均未检出耐药质粒。所有菌株均存在 76 个基因，其中包含 SPI-1 和 SPI-2 介导的 III 型分泌系统（T3SS）49 个基因、黏附（1 型菌毛、Agf 和 MisL 相关基因）13 个基因、铁摄取（耶尔森菌素）11 个基因、镁摄取 2 个基因和伤寒毒素 1 个基因。
儿童分离的 血清型和序列型少见；所有 血清型 对检测到的抗生素均敏感；T3SS、黏附、铁摄取、镁摄取和伤寒毒素与儿童 血清型 分离株的致病性有关。
