Artuyants Anastasiia, Chang Vanessa, Reshef Gabrielle, Blenkiron Cherie, Chamley Lawrence W, Leung Euphemia, Hisey Colin L
Hub for Extracellular Vesicle Investigations, University of Auckland, Auckland, New Zealand.
Department of Obstetrics and Gynaecology, University of Auckland, Auckland, New Zealand.
Methods Mol Biol. 2022;2436:183-192. doi: 10.1007/7651_2021_413.
The efficient production of extracellular vesicles (EVs) from adherent cells in vitro can be challenging when using conventional culture flasks. Issues such as low cell density leading to low EV yield, and the inability to completely remove bovine serum EVs without starvation contribute to this challenge. By comparison, the two-chamber CELLine adherent bioreactor can produce significantly more EVs with improved time, space, and resource efficiency. Furthermore, it is highly accessible and can continually produce EVs using long term cultures without the need for passaging. Lastly, the 10 kDa semipermeable, cellulose acetate membrane separating the cell and media chambers allows for the continual use of bovine serum in the media chamber while preventing bovine EVs from contaminating the conditioned media.
在体外使用传统培养瓶从贴壁细胞高效生产细胞外囊泡（EVs）可能具有挑战性。诸如细胞密度低导致EV产量低，以及不进行饥饿处理就无法完全去除牛血清来源的EVs等问题加剧了这一挑战。相比之下，双室CELLine贴壁生物反应器能够显著生产更多的EVs，同时提高时间、空间和资源利用效率。此外，它易于使用，并且无需传代就可以通过长期培养持续生产EVs。最后，分隔细胞室和培养基室的10 kDa半透性醋酸纤维素膜允许在培养基室中持续使用牛血清，同时防止牛来源的EVs污染条件培养基。
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