Husain T, Suhel M, Prasad S M, Singh V P
Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad, India.
Plant Physiology Laboratory, Department of Botany, C.M.P. Degree College, A Constituent Post Graduate College of University of Allahabad, Allahabad, India.
Plant Biol (Stuttg). 2022 Jun;24(4):652-659. doi: 10.1111/plb.13324. Epub 2021 Sep 7.
Chromium toxicity to crops is a major scientific problem of the present time. Thus, scientific attempts have been made for reducing chromium toxicity to crop plants. In this study, we examined the potential of ethylene (ET, 25 µM) and hydrogen sulphide (H S, 10 µM) to alleviate hexavalent chromium [Cr(VI), 50 µM] stress in two pulse crops, black bean and mung bean, by assessing physiological and biochemical attributes. Cr(VI) reduced shoot and root length in black bean and mung bean in comparison to the control. Plants had increased accumulation of oxidative stress markers, i.e. superoxide radicals (SOR), hydrogen peroxide (H O ) and lipid peroxidation (as malondialdehyde, MDA). The addition of AVG (an inhibitor of ET biosynthesis) and PAG (an inhibitor of H S biosynthesis) to Cr(VI)-treated plants further increased Cr(VI) toxicity, suggesting their endogenous levels are important for tolerating Cr(VI) toxicity. However, supplementation with either ET or H S alleviated Cr(VI) toxicity. Interestingly, ET did not rescue negative effects of PAG under Cr(VI) stress but NaHS rescued negative effect of AVG. Overall, results indicate that, although both ET and H S alleviate Cr(VI) stress, endogenous H S is better. Furthermore, H S appears to be a downstream signal for ET in alleviating Cr(VI) stress in these two pulse crops.
铬对农作物的毒性是当前一个重大的科学问题。因此，人们已展开科学尝试来降低铬对农作物的毒性。在本研究中，我们通过评估生理和生化特性，研究了乙烯（ET，25µM）和硫化氢（H₂S，10µM）缓解两种豆类作物黑豆和绿豆中六价铬[Cr(VI)，50µM]胁迫的潜力。与对照相比，Cr(VI)降低了黑豆和绿豆的地上部和根部长度。植物中氧化应激标志物即超氧自由基（SOR）、过氧化氢（H₂O₂）和脂质过氧化（以丙二醛，MDA表示）的积累增加。向经Cr(VI)处理的植物中添加AVG（一种ET生物合成抑制剂）和PAG（一种H₂S生物合成抑制剂）进一步增加了Cr(VI)毒性，表明它们的内源水平对于耐受Cr(VI)毒性很重要。然而，补充ET或H₂S均可缓解Cr(VI)毒性。有趣的是，在Cr(VI)胁迫下ET无法挽救PAG的负面影响，但NaHS可挽救AVG的负面影响。总体而言，结果表明，尽管ET和H₂S均可缓解Cr(VI)胁迫，但内源性H₂S效果更佳。此外，在缓解这两种豆类作物的Cr(VI)胁迫方面，H₂S似乎是ET的下游信号。
