Faculty of Pharmacy, Department of Pharmacognosy, Ain Shams University, Cairo, Egypt.
Faculty of Pharmacy and Biotechnology, Department of Pharmaceutical Biology, German University in Cairo, Cairo, Egypt.
Nat Prod Res. 2022 Aug;36(16):4187-4192. doi: 10.1080/14786419.2021.1961252. Epub 2021 Sep 7.
Renoprotectors are highly demanded due to environmental nephrotoxic factors. leaves extract alleviating effect was assessed in nephritic-induced rats by whole body shot dose of γ-radiation. Many biomarkers were detected using several assays. Renohistopathological examinations were performed. Moreover, the extract phytoconstituents were identified using spectroscopic analysis. anti-inflammatory activity of some compounds was examined using histamine release assay. Post-irradiation treatment with the extract significantly ameliorated all elevated biomarker levels. Creatinine and urea were adjusted, TGF-β/Smad signaling was suppressed causing down-regulation to microRNA-21. Nitric oxide, reactive oxygen species, glutathione and kidney injury molecule-1 were normalized in comparison with the γ-irradiated group. The renohistopathological analysis was consistent with the biochemical study. Phytochemical analysis resulted in the isolation of two diterpenoids (-methoxybutenolide clerodane diterpene and 16()-hydroxycleroda-3,13-dien-16,15-olide-2-one), aporphine alkaloid (anonaine) and flavonol (kaempferol-3--rutinoside). The latter two showed moderate anti-histaminic activities. Our results indicated that reduced oxidative stress and nephropathy in rats due to its anti-inflammatory principles.
由于环境肾毒性因素，人们对肾保护剂的需求很高。通过全身γ射线照射剂量，评估了 叶提取物对肾炎诱导大鼠的缓解作用。使用多种测定方法检测了许多生物标志物。进行了肾组织病理学检查。此外，还使用光谱分析鉴定了提取物的植物成分。使用组氨酸释放测定法检查了一些化合物的抗炎活性。与γ 射线照射组相比，辐照后用提取物治疗可显著改善所有升高的生物标志物水平。肌酐和尿素得到调整，TGF-β/Smad 信号被抑制，导致 microRNA-21 下调。与γ 射线照射组相比，一氧化氮、活性氧、谷胱甘肽和肾损伤分子 1 恢复正常。组织病理学分析与生化研究一致。植物化学分析导致两种二萜（-甲氧基丁烯内酯 clerodane 二萜和 16()-羟基 cleroda-3,13-二烯-16,15-内酯-2-one）、阿朴啡生物碱（anonaine）和类黄酮（山柰酚-3--芸香糖苷）的分离。后两种显示出中等的抗组胺活性。我们的结果表明， 由于其抗炎作用，减少了大鼠的氧化应激和肾病。
