University of California Davis School of Medicine, Sacramento, USA.
Sacramento VA Medical Center, 10535 Hospital Way, Mather, CA, 95655, USA.
Mol Biol Rep. 2021 Oct;48(10):6787-6796. doi: 10.1007/s11033-021-06678-2. Epub 2021 Sep 7.
CD146 (MCAM-melanoma cell adhesion molecule) is a cell surface adhesion molecule for Laminin 411. T cells expressing MCAM are mainly responsible for IL-17 production. IL-17 secreting T helper cells (Th17 cells) are critical for the pathogenesis of psoriatic arthritis (PsA). Here we hypothesized enrichment of CD146IL-17 memory T cells in PsA synovium and studied the association of CD146 expression and CD4IL-17 activated memory (CD11aCD45RO) T cells in synovial fluid and blood of PSA, rheumatoid arthritis (RA, a positive control) and osteoarthritis (OA) patients.
Hi-D FACS studies were done to identify IL-17 in CD4CD146CD45RO and CD8CD146CD45RO T cells.
We observed that effector CD146(MCAM) T cells are enriched at the synovial inflammation site in PsA.
As CD146 T cells are a key resource for IL-17 it is likely that the enrichment of these MCAM pathologic cells are critical for the disease process of PsA.
CD146（MCAM-黑色素瘤细胞黏附分子）是层粘连蛋白 411 的细胞表面黏附分子。表达 MCAM 的 T 细胞主要负责产生 IL-17。分泌 IL-17 的辅助性 T 细胞（Th17 细胞）对于银屑病关节炎（PsA）的发病机制至关重要。在这里，我们假设 CD146IL-17 记忆 T 细胞在 PsA 滑膜中富集，并研究了 CD146 表达与滑膜液和 PSA、类风湿关节炎（RA，阳性对照）和骨关节炎（OA）患者血液中 CD4IL-17 激活的记忆（CD11aCD45RO）T 细胞之间的关联。
采用 Hi-D FACS 研究鉴定 CD4CD146CD45RO 和 CD8CD146CD45RO T 细胞中的 IL-17。
我们观察到效应性 CD146（MCAM）T 细胞在 PsA 的滑膜炎症部位富集。
由于 CD146 T 细胞是 IL-17 的重要来源，因此这些 MCAM 病理性细胞的富集很可能对 PsA 的疾病进程至关重要。
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