Department of Botany, Punjabi University, Patiala, Punjab, India.
Department of Botany and Environmental Science, Sri Guru Granth Sahib World University, Fatehgarh Sahib, Punjab, India.
J Basic Microbiol. 2022 Sep;62(9):1156-1166. doi: 10.1002/jobm.202100285. Epub 2021 Sep 7.
Microalgae are a potential source of a wide range of food and novel value-added products. The versatility of microalgae to produce different kind of pigments is gaining interest as a sustainable source of natural carotenoids. Currently, commercial production of carotenoids from selected microalgae requires special culture conditions which are difficult to maintain. The present study has been undertaken to optimize culture conditions for growth and carotenoid production by a new isolate Scenedesmus quadricauda PUMCC 4.1.40. The results revealed that test organism produced 1.54 mg dry biomass/ml with a content of 40 μg carotenoids/mg dry biomass during stationary phase. The growth and carotenoid production was increased by 2.4-fold under combined optimized culture conditions. The optimized conditions were growth medium, Chu-10; pH 8.5; temperature, 30°C; nitrogen, 20 mM nitrate; phosphate, 0.22 mM; NaCl, 0.42 mM and blue light. Separation and identification of four important carotenoids through high-performance thin-layer chromatography (HPTLC) followed by purification using flash chromatography and quantification by HPLC revealed 23.8, 19.0, 6.5, and 4.0 μg astaxanthin, β-carotene, lutein, and canthaxanthin /mg dry biomass, respectively. The amount of total carotenoids (98 μg/mg dry biomass) containing 40% valuable astaxanthin and β-carotene produced under optimized conditions was significantly higher than control cultures. This justifies that S. quadricauda is a promising candidate for scale-up production of carotenoid.
微藻是多种食品和新型增值产品的潜在来源。微藻能够生产不同种类的色素，这使其作为天然类胡萝卜素的可持续来源引起了人们的兴趣。目前，从选定的微藻中商业生产类胡萝卜素需要特殊的培养条件，这些条件很难维持。本研究旨在优化新型分离株四尾栅藻 PUMCC 4.1.40 的生长和类胡萝卜素生产的培养条件。结果表明，在静止期，试验生物产生 1.54mg 干生物质/ml，其中含有 40μg 类胡萝卜素/mg 干生物质。在组合优化的培养条件下，生长和类胡萝卜素的生产增加了 2.4 倍。优化条件为生长培养基 Chu-10；pH8.5；温度 30°C；氮，20mM 硝酸盐；磷，0.22mM；NaCl，0.42mM 和蓝光。通过高效薄层色谱（HPTLC）分离和鉴定四种重要的类胡萝卜素，然后通过快速色谱法进行纯化，并通过 HPLC 进行定量，结果显示，虾青素、β-胡萝卜素、叶黄素和角黄素的含量分别为 23.8、19.0、6.5 和 4.0μg/mg 干生物质。在优化条件下，总类胡萝卜素（98μg/mg 干生物质）的含量为 40%，其中含有有价值的虾青素和β-胡萝卜素，明显高于对照培养物。这证明四尾栅藻是大规模生产类胡萝卜素的有前途的候选者。
