Sun Ke, Wu Jia-Hong, Bai Hua, Xu Sen-Lei, Xia Xue-Feng, Liu Yu-Chen, Lu Sheng-Feng, Gu Yi-Huang, Zhang Hong-Ru
College of Acupuncture-Moxibustion and Tuina, College of Health and Rehabilitation, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China.
College of Acupuncture-Moxibustion and Tuina, College of Health and Rehabilitation, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China; Key Laboratory of Acupuncture and Medicine of Ministry of Education, Nanjing University of Chinese Medicine.
Zhongguo Zhen Jiu. 2021 Sep 12;41(9):1023-8. doi: 10.13703/j.0255-2930.20200824-k0001.
To observe the effect of electroacupuncture (EA) on expression of interleukin (IL) -23/IL-17 axis and Toll-like receptor 4 (TLR4) in the infarcted tissue in rats with myocardial infarction (MI), and to explore the mechanism of EA on alleviating MI injury.
Forty male SD rats were randomly divided into a sham-operation group, a sham-operation plus EA group, a model group and an EA group, 10 rats in each group. The MI models were established by ligation of left anterior descending coronary artery in the model group and EA group, while only threading was performed in the sham-operation group and sham-operation plus EA group. The rats in the sham-operation plus EA group and EA group were treated with EA at "Neiguan" (PC 6), disperse-dense wave, 2 Hz/100 Hz, 2 mA, once a day, 20 min each time, for 3 days. After the intervention, the ejection fraction (EF) was measured by echocardiography to evaluate the cardiac function; the infarct area was measured by TTC staining; the HE staining was used to observe the morphological changes of myocardial tissue; the levels of IL-23 and IL-17 in infarcted tissue were detected by ELISA; the protein expression of TLR4 in infarcted tissue was detected by Western blot.
Compared with the sham-operation group, the EF was decreased (<0.01), the infarct area was increased (<0.01), the myocardial fiber injury was obvious, accompanied by inflammatory cell infiltration, and the contents of IL-23, IL-17 and the expression of TLR4 in infarcted tissue were increased in the model group (<0.01). Compared with the model group, the EF was increased (<0.05), the infarct area was reduced (<0.05), the myocardial fiber injury was significantly improved, the inflammatory cell infiltration was reduced, and the contents of IL-23, IL-17 and TLR4 expression in infarcted tissue were decreased in the EA group (<0.05).
EA may alleviate the excessive inflammatory response after MI by inhibiting the expression of IL-23/IL-17 axis in MI rats, and TLR4 may be involved during the process.
观察电针（EA）对心肌梗死（MI）大鼠梗死组织中白细胞介素（IL）-23/IL-17轴及Toll样受体4（TLR4）表达的影响，探讨EA减轻MI损伤的机制。
40只雄性SD大鼠随机分为假手术组、假手术加EA组、模型组和EA组，每组10只。模型组和EA组采用结扎左冠状动脉前降支的方法建立MI模型，假手术组和假手术加EA组仅穿线。假手术加EA组和EA组大鼠于“内关”（PC 6）穴行电针治疗，疏密波，2 Hz/100 Hz，2 mA，每日1次，每次20 min，共3天。干预后，采用超声心动图测量射血分数（EF）以评估心功能；采用TTC染色测量梗死面积；采用HE染色观察心肌组织形态学变化；采用ELISA法检测梗死组织中IL-23和IL-17水平；采用Western blot法检测梗死组织中TLR4蛋白表达。
与假手术组比较，模型组EF降低（<0.01），梗死面积增大（<0.01），心肌纤维损伤明显，伴有炎性细胞浸润，梗死组织中IL-23、IL-17含量及TLR4表达升高（<0.01）。与模型组比较，EA组EF升高（<0.05），梗死面积减小（<0.05），心肌纤维损伤明显改善，炎性细胞浸润减少，梗死组织中IL-23、IL-17含量及TLR4表达降低（<0.05）。
EA可能通过抑制MI大鼠IL-23/IL-17轴的表达减轻MI后的过度炎症反应，TLR4可能参与此过程。
