Oliveira Adriana Paulo de Sousa, Assemany Paula, Ribeiro Júnior José Ivo, Covell Lidiane, Nunes-Nesi Adriano, Calijuri Maria Lúcia
Department of Civil Engineering, Federal University of Viçosa, Viçosa, MG, Brazil.
Department of Environmental Engineering, Federal University of Lavras, Lavras, MG, Brazil.
J Environ Manage. 2021 Dec 1;299:113668. doi: 10.1016/j.jenvman.2021.113668. Epub 2021 Sep 4.
This study aimed to evaluate the simultaneous interferences of Cu and Zn found in swine wastewater (SW) in the development of microalgae considering real conditions of cultivation in high rate algal ponds (HRAPs). Ten HRAPs on a pilot scale were fed with SW with different mixtures of Cu (0.5-3.0 mg/L) and Zn (5.0-25.0 mg/L). The interferences of these metals in removing nutrients (N-NH and soluble phosphorus (Ps)) from the SW were determined. In addition, this study evaluated the effects on biomass growth and biochemical composition. Chlorella sp. was dominant in all HRAPs and the condition that potentiated its growth occurred in medium containing 1.8 mg Cu/L + 15.0 mg Zn/L, while higher concentrations conferred inhibition. Only Cu compromised the removal rates of N-NH while the effects of Zn were not significant. Contrary, Zn interfered with Ps removal rates, but the impact of Cu was not significant. The greatest Cu applications increased the protein levels by biomass (50.5-55.2 %). Carbohydrate accumulation was favored by conditions that inhibited the development of microalgae due to either limitation or excess of metals. Copper and Zn compromised the levels of lipids, and the control treatment had the highest content (24.5 %). The presence of Cu and Zn changed the dynamics of HRAPs regarding nutrient removal, productivity, and biochemical composition of the biomass.
本研究旨在考虑在高率藻塘（HRAPs）中的实际培养条件，评估猪废水（SW）中发现的铜和锌对微藻生长发育的同时干扰。十个中试规模的HRAPs被投喂含有不同铜（0.5 - 3.0毫克/升）和锌（5.0 - 25.0毫克/升）混合物的SW。测定了这些金属对从SW中去除营养物质（N - NH和可溶性磷（Ps））的干扰。此外，本研究评估了对生物量生长和生化组成的影响。小球藻在所有HRAPs中占主导地位，促进其生长的条件出现在含有1.8毫克铜/升 + 15.0毫克锌/升的培养基中，而较高浓度则产生抑制作用。只有铜会影响N - NH的去除率，而锌的影响不显著。相反，锌会干扰Ps的去除率，但铜的影响不显著。最大量的铜应用使生物量中的蛋白质水平提高了（50.5 - 55.2%）。由于金属限制或过量而抑制微藻生长发育的条件有利于碳水化合物的积累。铜和锌会降低脂质水平，对照处理的脂质含量最高（24.5%）。铜和锌的存在改变了HRAPs在营养物质去除、生产力和生物量生化组成方面的动态。
