Guangdong Provincial Research Centre for Environment Pollution Control and Remediation Materials, Department of Ecology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Guangdong Provincial Research Centre for Environment Pollution Control and Remediation Materials, Department of Ecology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China; State Environmental Protection Key Laboratory of Integrated Surface Water Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China.
J Hazard Mater. 2021 Aug 15;416:125894. doi: 10.1016/j.jhazmat.2021.125894. Epub 2021 Apr 15.
The chemistry of root cell wall of rice could be changed by inoculation of arbuscular mycorrhizal fungi (AMF). Hydroponic experiments were conducted to investigate the roles of such changes on cadmium (Cd) uptake and distribution in rice. Results showed that inoculation of AM fungus Rhizophagus intraradices (RI) significantly enhanced (p < 0.05) shoot biomass, plant height and root length of rice, and decreased Cd concentration in shoot and root under Cd stress. Moreover, Cd in root was mainly found in pectin and hemicellulose 1 (HC1) components of root cell wall. Inoculation of RI increased the levels of pectin, HC1 and lignin content, accompanied by the increments of L-phenylalanine ammonia-lyase (PAL) and pectin methylesterase (PME) activities. Results of Fourier transform infrared spectroscopy further showed that more hydroxyl and carboxyl groups in root cell wall were observed in mycorrhizal treatment, compared with control. This study demonstrates that cell wall components could be the locations for Cd fixation, which reduced Cd transportation from root to shoot. Inoculation of AMF may remodel root cell wall biosynthesis and affect the characteristics of Cd fixation. The entering and fixing mechanisms should be further studied.
接种丛枝菌根真菌（AMF）可以改变水稻根细胞壁的化学性质。通过水培实验研究了这些变化对水稻镉（Cd）吸收和分布的影响。结果表明，接种 AM 真菌 Rhizophagus intraradices（RI）显著增强了（p<0.05）Cd 胁迫下水稻的地上生物量、株高和根长，并降低了地上部和根部的 Cd 浓度。此外，Cd 主要存在于根细胞壁的果胶和半纤维素 1（HC1）成分中。RI 的接种增加了果胶、HC1 和木质素的含量，同时伴随着苯丙氨酸解氨酶（PAL）和果胶甲酯酶（PME）活性的增加。傅里叶变换红外光谱的结果进一步表明，与对照相比，菌根处理的根细胞壁中观察到更多的羟基和羧基。本研究表明，细胞壁成分可能是 Cd 固定的位置，从而减少了 Cd 从根部向地上部的运输。接种 AMF 可能会重塑根细胞壁的生物合成，并影响 Cd 固定的特性。进入和固定的机制还需要进一步研究。
