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用于降低纺织工业废水污染负荷的解脂诺卡氏菌及用于脂肪酶结构研究的SWISS模型。

Lipolytic Nocardiopsis for reduction of pollution load in textile industry effluent and SWISS model for structural study of lipase.

作者信息

Patel Gayatriben B, Rakholiya Parita, Shindhal Toral, Varjani Sunita, Tabhani N M, Shah Kamleshkumar R

机构信息

Department of Biotechnology, Shree Maneklal M Patel Institute of Science and Research, Kadi Sarva Vishwavidyalaya, Gandhinagar, Gujarat 382015, India.

Department of Biotechnology, Shree Maneklal M Patel Institute of Science and Research, Kadi Sarva Vishwavidyalaya, Gandhinagar, Gujarat 382015, India; Gujarat Pollution Control Board, Gandhinagar, Gujarat 382010, India.

出版信息

Bioresour Technol. 2021 Dec;341:125673. doi: 10.1016/j.biortech.2021.125673. Epub 2021 Aug 2.

Abstract

The aim of this work was to study the reduction of pollution load in the textile industry effluent by lipase producing Nocardiopsis alba. Nocardiopsis alba was isolated from the soap-stock of an oil refinery located in North Gujarat, India. Lipase 2 (EC 3.1.1.3) protein sequence was identified from Nocardiopsis alba and the 3D model was predicted by SWISS Modeling. Lipolytic Nocardiopsis alba showed 84 ± 0.3 %, 75 ± 0.4 %, and 81 ± 0.3 % reduction of Chemical Oxygen Demand, Biochemical Oxygen Demand and Total Solids, respectively when was grown in textile industrial wastewater at 37 ± 1 °C, 120 rpm for 15 days. The findings of this research offer potential environmental benefits along with socio-economic benefits as well if implemented suitably.

摘要

这项工作的目的是研究产脂肪酶的白色诺卡氏菌对纺织工业废水污染负荷的降低作用。白色诺卡氏菌是从印度古吉拉特邦北部一家炼油厂的皂脚中分离出来的。从白色诺卡氏菌中鉴定出脂肪酶2(EC 3.1.1.3)蛋白序列,并通过SWISS建模预测了其三维模型。当在37±1°C、120 rpm的条件下于纺织工业废水中培养15天时,产脂肪的白色诺卡氏菌分别使化学需氧量、生化需氧量和总固体减少了84±0.3%、75±0.4%和81±0.3%。如果适当实施,本研究结果将带来潜在的环境效益以及社会经济效益。

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