Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), P. O. Box 577, Jhang Road, Faisalabad, Pakistan.
Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), P. O. Box 577, Jhang Road, Faisalabad, Pakistan.
Water Res. 2014 Jul 1;58:152-9. doi: 10.1016/j.watres.2014.03.064. Epub 2014 Apr 2.
Textile effluent is one of the main contributors of water pollution and it adversely affects fauna and flora. Constructed wetland is a promising approach to remediate the industrial effluent. The detoxification of industrial effluent in a constructed wetland system may be enhanced by applying beneficial bacteria that are able to degrade contaminants present in industrial effluent. The aim of this study was to evaluate the influence of inoculation of textile effluent-degrading endophytic bacteria on the detoxification of textile effluent in a vertical flow constructed wetland reactor. A wetland plant, Typha domingensis, was vegetated in reactor and inoculated with two endophytic bacterial strains, Microbacterium arborescens TYSI04 and Bacillus pumilus PIRI30. These strains possessed textile effluent-degrading and plant growth-promoting activities. Results indicated that bacterial inoculation improved plant growth, textile effluent degradation and mutagenicity reduction and were correlated with the population of textile effluent-degrading bacteria in the rhizosphere and endosphere of T. domingensis. Bacterial inoculation enhanced textile effluent-degrading bacterial population in rhizosphere, root and shoot of T. domingensis. Significant reductions in COD (79%), BOD (77%) TDS (59%) and TSS (27%) were observed by the combined use of plants and bacteria within 72 h. The resultant effluent meets the wastewater discharge standards of Pakistan and can be discharged into the environment without any risks. This study revealed that the combined use of plant and endophytic bacteria is one of the approaches to enhance textile effluent degradation in a constructed wetland system.
纺织废水是水污染的主要来源之一，它对动植物区系有不利影响。人工湿地是一种很有前途的方法来修复工业废水。通过应用能够降解工业废水中污染物的有益细菌，可以增强人工湿地系统中工业废水的解毒效果。本研究的目的是评估接种纺织废水降解内生细菌对垂直流人工湿地反应器中纺织废水解毒的影响。在反应器中种植了湿地植物香蒲，并接种了两种内生细菌，即树突微杆菌 TYSI04 和短小芽孢杆菌 PIRI30。这些菌株具有纺织废水降解和促进植物生长的活性。结果表明，细菌接种提高了植物的生长、纺织废水的降解和致突变性的降低，这与香蒲根际和内生区纺织废水降解细菌的种群有关。细菌接种增加了香蒲根际、根和茎中纺织废水降解细菌的种群。在 72 小时内，植物和细菌的联合使用使 COD（79%）、BOD（77%）、TDS（59%）和 TSS（27%）显著降低。产生的废水符合巴基斯坦的废水排放标准，可以直接排放到环境中而不会带来任何风险。本研究表明，植物和内生细菌的联合使用是提高人工湿地系统中纺织废水降解的方法之一。
