Ji Li, Ma Li-Xin, Cheng Zheng-Lei, Zhu Qing-Chao, Zhang Yan, Yang Yu-Chun, Yang Li-Xue
Ministry of Education Key Laboratory of Sustainable Forest Ecosystem Management, School of Forestry, Northeast Forestry University, Harbin 150040, China.
Jilin Academy of Forestry, Changchun 130033, China.
Ying Yong Sheng Tai Xue Bao. 2020 Aug;31(8):2491-2499. doi: 10.13287/j.1001-9332.202008.005.
In this study, we examined the characteristics and influence mechanism of soil extracellular enzyme activity (EEA) and enzymatic stoichiometry in different soils in forests at different altitudes (750-1420 m) in Aokelidui Mountains in the north of the Greater Khingan Mountains. The results showed that altitude, season and their interactions significantly affected the activities of β-glucosidase (BG), β-1,4-N-acetylglucosaminidase (NAG), L-leucine aminopeptidase (LAP), and acid phosphatase (AP). In May, BG and NAG activities gradually increased with increasing altitude, while AP activities increased first and then decreased with increasing altitude. In July, NAG activity increased with altitude, while AP activity increased first and then decreased. In September, NAG activity changed significantly in different altitudes, with the highest activity at 1420 m (124.22 nmol·h·g). With the increases of altitude, ln(BG): ln(NAG+LAP) showed a decreasing trend. Except for the altitude of 830 m, stoichiometric ratio in all altitudes was the highest in July. The ratio of logarithmic conversion of soil C, N, and P invertase activity was 1:1.25:0.82. Altitude and soil temperature were the main factors affecting soil extracellular enzyme activities. There was a significant positive correlation between soil temperature and BG, NAG, and AP. Enzymatic stoichiometry ln(BG):ln(NAG+LAP) and ln(NAG+LAP):ln(AP) showed significant positive and negative correlations with soil pH, and had a negative and positive relationship with DOC. The ratio of ln(BG):ln(AP) was greatly affected by soil bulk density.
在本研究中，我们调查了大兴安岭北部奥克里堆山不同海拔（750 - 1420米）森林中不同土壤的土壤胞外酶活性（EEA）和酶化学计量特征及其影响机制。结果表明，海拔、季节及其相互作用显著影响β - 葡萄糖苷酶（BG）、β - 1,4 - N - 乙酰氨基葡萄糖苷酶（NAG）、L - 亮氨酸氨基肽酶（LAP）和酸性磷酸酶（AP）的活性。5月，BG和NAG活性随海拔升高逐渐增加，而AP活性随海拔升高先增加后降低。7月，NAG活性随海拔升高而增加，而AP活性先增加后降低。9月，NAG活性在不同海拔间变化显著，在1420米处活性最高（124.22 nmol·h·g）。随着海拔升高，ln（BG）:ln（NAG + LAP）呈下降趋势。除830米海拔外，所有海拔的化学计量比在7月最高。土壤碳、氮、磷转化酶活性的对数转换比为1:1.25:0.82。海拔和土壤温度是影响土壤胞外酶活性的主要因素。土壤温度与BG、NAG和AP之间存在显著正相关。酶化学计量ln（BG）:ln（NAG + LAP）和ln（NAG + LAP）:ln（AP）与土壤pH呈显著正相关和负相关，与溶解性有机碳（DOC）呈负相关和正相关关系。ln（BG）:ln（AP）的比值受土壤容重影响较大。
