Zhang Min-Huan, Wang Jian-Cheng, Yang Hong-Lan, Zhang Dao-Yuan, Deng Peng-Cheng, Cui Zhi-Jun
College of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, China.
State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Ying Yong Sheng Tai Xue Bao. 2020 Aug;31(8):2558-2566. doi: 10.13287/j.1001-9332.202008.002.
We examined the physiological response of 1-year-old branches of 37 Xinjiang wild walnut germplasm resources in Gongliu wild walnut forest under cold temperatures (-20 ℃ and 4 ℃) for 12 hours, compared the responses with that of . The relative conductivity (REC), free proline (PRO), soluble sugar (SS), malondialdehyde (MDA) content and peroxidase (POD) activity were measured in an artificial climate chamber by simulating spring low temperature. Subordinate function and principal component analysis were used to evaluate the physiological response of walnut germplasm to low temperature. The results showed that the REC, PRO, SS, MDA content and POD activity of Xinjiang wild walnut were increased. By evaluating the relationship between low temperature resistance and habitat, we found that cold tolerance level was middle valley > east valley > west valley > general valley. Xinjiang wild walnut showed stronger cold tolerance than . Seven germplasm with cold resistance were selected from the total 37 wild walnut germplasm in Xinjiang, which provided a reference for improving walnut varieties and their responses to sudden weather change in late spring and other growing stages.
我们研究了巩留野核桃林中37份新疆野生核桃种质资源1年生枝条在低温（-20℃和4℃）下12小时的生理响应，并将其与……的响应进行了比较。通过模拟春季低温，在人工气候箱中测定了相对电导率（REC）、游离脯氨酸（PRO）、可溶性糖（SS）、丙二醛（MDA）含量和过氧化物酶（POD）活性。采用隶属函数和主成分分析方法评价核桃种质对低温的生理响应。结果表明，新疆野生核桃的REC、PRO、SS、MDA含量和POD活性均有所增加。通过评估抗寒性与栖息地之间的关系，我们发现耐寒性水平为中谷＞东谷＞西谷＞普通山谷。新疆野生核桃表现出比……更强的耐寒性。从新疆37份野生核桃种质中筛选出7份抗寒种质，为核桃品种改良及其对春末等生长阶段突发气候变化的响应提供了参考。
