Song Ya-Ting, Ma Kai, Zhao Yu, Han Li-Qun, Liu Li-Qiang
College of Horticulture, Xinjiang Agricultural University, Urumqi, China.
Xinjiang Key Laboratory of Genome Research and Genetic Improvement of Specialty Fruits and Vegetables, Xinjiang Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Xinjiang Regional Scientific Observatory and Experiment Station of Fruit Trees, Ministry of Agriculture, Urumqi, China.
Front Genet. 2024 May 13;15:1399721. doi: 10.3389/fgene.2024.1399721. eCollection 2024.
MYC transcription factors are the basic regulators of the jasmonic acid signaling pathway and play important roles in plant growth and development and the response to adverse stress. In recent years, severe winter freezing and late spring frost in the main planting area of walnut in Xinjiang have affected the growth and development of walnut, which has become a prominent problem restricting walnut production. Xinjiang wild walnut is the only remaining wild species of walnuts in China, which contains a lot of genes with excellent traits, and is important for the cultivation and breeding. In this paper, the physicochemical properties and bioinformatics of MYC transcription factor members in walnut were analyzed, and the nine MYC were screened from the transcriptome data under low temperature stress. At last, we study the subcellular localizations and the expression patterns of the nine MYC members in Xinjiang wild walnut. The results revealed that 30 MYC members were identified from published walnut whole-genome data, and their evolutionary relationships with Arabidopsis and poplar were divided into six groups according to clustering analysis, among which had high homology with , which is induced by jasmonic acid in response to low-temperature stress. Walnut MYC members are unevenly distributed on 12 chromosomes. The prediction of promoter cis-acting elements of walnut MYC transcription factor family members revealed that cis-acting elements related to jasmonic acid and lowtemperature stress were the ones with the greatest number of members, with 12. In addition, all nine MYC family members in Xinjiang wild walnut plants responding to low-temperature stress exhibited strong fluorescence responses in the nucleus. The expression levels of these members in response to low-temperature stress revealed that were highly expressed, and it was hypothesized that might play a key role in the response to lowtemperature stress. The results of this study provide a theoretical basis for further research on the functional mechanisms of the MYC transcription factor family members in walnut.
MYC转录因子是茉莉酸信号通路的基本调节因子，在植物生长发育和逆境胁迫响应中发挥重要作用。近年来，新疆核桃主产区冬季严寒和晚春霜冻影响了核桃的生长发育，已成为制约核桃生产的突出问题。新疆野核桃是我国仅存的核桃野生种，含有许多优良性状基因，对核桃栽培和育种具有重要意义。本文分析了核桃MYC转录因子成员的理化性质和生物信息学，并从低温胁迫下的转录组数据中筛选出9个MYC。最后，我们研究了新疆野核桃中9个MYC成员的亚细胞定位和表达模式。结果表明，从已发表的核桃全基因组数据中鉴定出30个MYC成员，根据聚类分析，它们与拟南芥和杨树的进化关系分为6组，其中与茉莉酸诱导响应低温胁迫的基因具有较高同源性。核桃MYC成员在12条染色体上分布不均。对核桃MYC转录因子家族成员启动子顺式作用元件的预测表明，与茉莉酸和低温胁迫相关的顺式作用元件成员数量最多，为12个。此外，新疆野核桃植株中9个MYC家族成员在低温胁迫下均在细胞核中表现出强烈的荧光反应。这些成员在低温胁迫下的表达水平表明，部分成员高表达，推测某些成员可能在低温胁迫响应中起关键作用。本研究结果为进一步研究核桃MYC转录因子家族成员的功能机制提供了理论依据。
